Abstract
BLOOD pyruvic acid estimations were being made in this laboratory, and it was important to test the specificity of the method employed. The acid was extracted as the hydrazone by the method of Friedemann and Haugen1 (using toluene for the extraction) and the hydrazones chromatographed under the conditions which Altmann et al.2 had found successful.
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References
Friedemann, T. E., and Haugen, G. E., J. Biol. Chem., 147, 415 (1943).
Altmann, S. M., Crook, E. M., and Datta, S. P., Biochem. J., 49, lxiii (1951).
Cavallini, D., Frontali, N., and Toschi, G., Nature, 163, 568 (1949).
Cavallini, D., Frontali, N., and Toschi, G., Nature, 164, 792 (1949).
Long, C., Biochem. J., 38, 447 (1944).
Bueding, E., and Wortis, H., J. Biol. Chem., 133, 585 (1940).
Lu, G. D., Biochem. J., 33, 249 (1939).
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TURNOCK, D. Paper Chromatography of Keto-Acids. Nature 172, 355–356 (1953). https://doi.org/10.1038/172355b0
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DOI: https://doi.org/10.1038/172355b0
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