Abstract
Hilton and Taylor1 obtained highly motile cultures of Bact. coli D 433 and of four other Bact. coli O group 111 strains by repeated subculture in nutrient broth and semi-solid agar and incubation at 22° C.; the period of passage required for this change was three to four weeks in semi-solid agar and slightly longer in broth. In this Laboratory, Bact. coli D 433 and other Bact. coli O group 111 strains have been found to develop a high degree of motility in 18–24 hr. under the following conditions. The strains are inoculated by a straight wire into 3 ml. of semi-solid agar (5 parts digest ox-heart broth + 1 part nutrient digest ox-heart agar, final pH. 7.4–7.6) in small screw-capped bottles (bijou type). A microscopic examination for motility is made of the surface growth which develops in 18–24 hr. at 20–22° C. The method has been used satisfactorily for routine motility testing of these organisms when subcultured direct from stock strains (stored at room temperature for 6–22 months in nutrient digest ox-heart agar stabs or on slopes of Loeffler's serum medium) or from fresh subcultures on MacConkey's medium.
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Hilton, R. E., and Taylor, J., Nature, 167, 359 (1951).
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WRIGHT, J. Motility Testing of Bact. coli O Group III Strains. Nature 167, 732 (1951). https://doi.org/10.1038/167732b0
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DOI: https://doi.org/10.1038/167732b0
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