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Progenitor Cell Mobilization

Enumeration of HPC in mobilized peripheral blood with the Sysmex SE9500 predicts final CD34+ cell yield in the apheresis collection

Bone Marrow Transplantation volume 25, pages 1157–1164 (2000)Cite this article

Abstract

Enumeration of CD34+ cells in the peripheral blood before apheresis predicts the quantity of those cells collected, although the cytometric techniques used are complex and expensive. We found that a subpopulation of lysis-resistant cells in the peripheral blood, identified by the Sysmex SE9500 and designated as HPC, can serve as a surrogate marker predictive of the yield of CD34+ cells. Spearman's rank statistics were used to examine the correlation between WBC, MNC, HPC and CD34+ cells in the peripheral blood and final CD34+ cell yield for 112 samples of peripheral blood and matching apheresis collections from 66 patients and donors. The results indicate that WBC and MNC in the peripheral blood were poor predictors of CD34 content, while HPC gave a correlation coefficient of 0.62. The positive predictive values of different cutoff levels of HPC in the peripheral blood ranging from 5 to 50 × 106/l increased from 0.80 to 0.93 when the target collection was 1 × 106cells/kg. However, for patients with HPC levels below various cutoff levels, the proportion of the collections not reaching that target goal ranged between 0.36 and 0.43, indicating that most collections will still exceed the target goal of CD34+ cells. When the target collection was 2.5 × 106 CD34+ cells/kg, the positive predictive value was lower and negative predictive value was higher. Bone Marrow Transplantation (2000) 25, 1157–1164.

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References

  1. Bensinger WI, Longin K, Appelbaum FR et al. Peripheral blood stem cells (PBSCs) collected after recombinant granulocyte colony stimulating factor (rhG-GSF): an analysis offactors correlating with the tempo of engraftment aftertransplantation Br J Haematol 1994 87: 825–831

    Article  CAS  PubMed  Google Scholar 

  2. Perez-Simon JA, Caballero MD, Corral M et al. Minimal number of circulating CD34+ cells to ensure successful leukapheresis and engraftment in autologous peripheral blood progenitor cell transplantation Transfusion 1998 38: 385–391

    Article  CAS  PubMed  Google Scholar 

  3. Bensinger WI, Singer J, Appelbaum FR et al. Autologous transplantation with peripheral blood mononuclear cellscollected after administration of recombinant granulocytestimulating factor Blood 1993 81: 3158–3163

    CAS  PubMed  Google Scholar 

  4. Bender JG, To LB, Williams S, Schwartzberg LS . Defining a therapeutic dose of peripheral blood stem cells J Hematother 1992 1: 329–341

    Article  CAS  PubMed  Google Scholar 

  5. Grigg AP, Roberts AW, Raunow H et al. Optimizing dose and scheduling of G-CSF (granulocyte colony-stimulating factor) for mobilization and collection of peripheral blood progenitor cells in normal volunteers Blood 1995 86: 4437–4445

    CAS  PubMed  Google Scholar 

  6. Tjonnfjord GE, Steen R, Evensen SA et al. Characterization of CD34+ peripheral blood cells from healthy adults mobilized by recombinant human granulocyte colony-stimulating factor Blood 1994 84: 2795–2801

    CAS  PubMed  Google Scholar 

  7. Stewart AK, Imrie K, Keating A et al. Optimizing the CD34+ and CD34+thy-1+ stem cell content of peripheral blood collections Exp Hematol 1995 23: 1619–1627

    CAS  PubMed  Google Scholar 

  8. Demirer T, Buckner CD, Appelbaum FR et al. Rapid engraftment after autologous transplantation utilizing marrow and recombinant granulocyte colony-stimulating factor-mobilized peripheral blood stem cells in patients with acute myelogenous leukemia Bone Marrow Transplant 1995 15: 915–922

    CAS  PubMed  Google Scholar 

  9. Ho AD, Gluck S, Germond C et al. Optimal timing for collections of blood progenitor cells following induction chemotherapy and granulocyte–macrophage colony-stimulating factor for autologous transplantation in advanced breast cancer Leukemia 1993 7: 1738–1746

    CAS  PubMed  Google Scholar 

  10. Elliott C, Samson DM, Armitage S et al. When to harvest peripheral-blood stem cells after mobilization therapy: prediction of CD34-positive cell yield by preceding day CD34-positive cell concentration in peripheral blood J Clin Oncol 1996 14: 970–973

    Article  CAS  PubMed  Google Scholar 

  11. Schots R, Riet IV, Damiaens S et al. The absolute number of circulating CD34+ cells predicts the number of hematopoietic stem cells that can be collected by apheresis Bone Marrow Transplant 1996 17: 509–515

    CAS  PubMed  Google Scholar 

  12. Passos-Coelho JL, Braine HG, Davis JM et al. Predictive factors for peripheral-blood progenitor-cell collections using a single large-volume leukapheresis after cyclophosphamide and granulocyte–macrophage colony-stimulating factor mobilization J Clin Oncol 1995 13: 705–714

    Article  CAS  PubMed  Google Scholar 

  13. Yu J, Leisenring W, Rowley SD et al. The predictive value of white cell or CD34+ cell count in peripheral blood for timing apheresis and maximizing yield Transfusion 1999 39: 442–450

    Article  CAS  PubMed  Google Scholar 

  14. Takekawa K, Yamane T, Suzuki K et al. Identification of hematopoietic stem cells by the SE-9000TM automated hematology analyzer in peripheral blood stem cell harvest samples Acta Hematologica 1997 98: 54–55

    Article  CAS  Google Scholar 

  15. Takekawa K, Yamane T, Suzuki K et al. Identification of hematopoietic stem cells by the SE-9000TM automated hematology analyzer in peripheral blood stem cell harvesting sample Blood 1996 88: 250b

    Google Scholar 

  16. Yamane T, Takekawa K, Tatsumi N . Possibility of identification of hematopoietic stem cells using a conventional blood cell counter Eur J Haematol 1995 55: 207–208

    Article  CAS  PubMed  Google Scholar 

  17. Bensinger WI, Appelbaum FR, Rowley S et al. Factors that influence collection and engraftment of autologous peripheral-blood stem cells J Clin Oncol 1995 13: 2547–2555

    Article  CAS  PubMed  Google Scholar 

  18. Conover WJ . Practical Non-parametric Statistics Wiley: New York 1980

    Google Scholar 

  19. Weisberg S . Applied Linear Regression Wiley: New York 1985

    Google Scholar 

  20. Leisenring W, Pepe MS, Longton G . A marginal regression modeling framework for evaluation of medical diagnostic tests Stat Med 1997 16: 1263–1281

    Article  CAS  PubMed  Google Scholar 

  21. Leisenring W, Alonzo T, Pepe MS . Comparisons of predictive values of binary medical diagnostic tests for paired designs Biometrics (in press)

  22. Haas R, Mohle R, Fruhauf S et al. Patient characteristics associated with successful mobilizing and autografting of peripheral blood progenitor cells in malignant lymphoma Blood 1994 83: 3787–3794

    CAS  PubMed  Google Scholar 

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Acknowledgements

We thank the staff of the Apheresis Unit, the Hematology Laboratory and the Cryobiology Laboratory of the Fred Hutchinson Cancer Research Center for performing the apheresis procedures, HPC and other blood cell counts and CD34+ cell counts. We also thank Sysmex Corporation of America for providing access to the analyzer and the supplies needed to conduct this work. Support for this project was provided in part by grant numbers CA18029, CA15704, and DK56465 from the National Institutes of Health, Bethesda, MD, and by the Sysmex Corporation.

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  1. Clinical Research Division, Division of Oncology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, USA

    J Yu, W Leisenring, W Fritschle, S Heimfeld, H Shulman, WI Bensinger, LA Holmberg & SD Rowley

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  1. J Yu
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Yu, J., Leisenring, W., Fritschle, W. et al. Enumeration of HPC in mobilized peripheral blood with the Sysmex SE9500 predicts final CD34+ cell yield in the apheresis collection. Bone Marrow Transplant 25, 1157–1164 (2000). https://doi.org/10.1038/sj.bmt.1702406

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