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L.S., F.T., F.I. and F.D.G. are authors on a patent application WO, 2005/012913.
Supplementary information
Supplementary Figure 1
SNAP-HAGFP signal redistribution during apoptosis.
Supplementary Figure 2
SNAP-HAGFP cleavage occurs early during apoptosis.
Supplementary Figure 3
Active caspase 3 cleaves SNAP-HAGFP.
Supplementary Figure 4
SNAP-mutHAGFP (DVPD of HA replaced by DVPE) is not cleavable and does not react with the anti-HA antibody.
Supplementary Figure 5
HA cleavage occurs irrespective of the nature and the location of the construct.
Supplementary Video 1
Live cell imaging of HA cleavage.
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Schembri, L., Dalibart, R., Tomasello, F. et al. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods 4, 107–108 (2007). https://doi.org/10.1038/nmeth0207-107
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DOI: https://doi.org/10.1038/nmeth0207-107
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