Abstract
Cytosine methylation of genomic DNA controls gene expression and maintains genome stability. How a specific DNA sequence is targeted for methylation by a methyltransferase is largely unknown. Here, we show that histone H3 tails lacking lysine 4 (K4) methylation function as an allosteric activator for methyltransferase Dnmt3a by binding to its plant homeodomain (PHD). In vitro, histone H3 peptides stimulated the methylation activity of Dnmt3a up to 8-fold, in a manner reversely correlated with the level of K4 methylation. The biological significance of allosteric regulation was manifested by molecular modeling and identification of key residues in both the PHD and the catalytic domain of Dnmt3a whose mutations impaired the stimulation of methylation activity by H3 peptides but not the binding of H3 peptides. Significantly, these mutant Dnmt3a proteins were almost inactive in DNA methylation when expressed in mouse embryonic stem cells while their recruitment to genomic targets was unaltered. We therefore propose a two-step mechanism for de novo DNA methylation – first recruitment of the methyltransferase probably assisted by a chromatin- or DNA-binding factor, and then allosteric activation depending on the interaction between Dnmt3a and the histone tails – the latter might serve as a checkpoint for the methylation activity.
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Introduction
In plants and vertebrate animals, regulation of gene expression is mainly accomplished through the modification of DNA and its associated histones. Cytosine methylation is the best-known type of modification found in the genomes of these organisms. DNA cytosine methylation together with histone modifications present two profound epigenetic regulatory platforms essential for various developmental and disease processes 1.
In mammals, DNA methylation patterns are established by the action of two de novo methyltransferases Dnmt3a and Dnmt3b during embryonic development and gametogenesis and then stably propagated by a maintenance methyltransferase Dnmt1 2. Missense mutations in DNMT3A are associated with acute myeloid leukemia 3. A fundamental question concerns how a specific sequence is selectively methylated by the de novo enzymes in cells. Evidence for cross-talk between DNA methylation and histone modification has been found in various contexts of transcriptional regulation and chromatin functions 4, 5, 6, 7, 8. In the filamentous fungus Neurospora crassa, either the replacement of histone H3 lysine 9 (H3K9) with other amino acids or deletion of the sole H3K9 methyltransferase DIM5 results in the loss of DNA methylation 9. In mouse embryonic stem (ES) cells, deficiency in the histone H3K9 methyltransferases Suv39h1-Suv39h2 is associated with hypomethylation at a subset of repeat elements 10. In the epigenome landscape, cytosine methylation is under-represented in regions containing histone H3K4 trimethylation 11, 12. These observations, mostly correlative, suggest that chromatin cues might be a determinant of DNA methylation in mammals. Interestingly, all Dnmt3 members possess a plant homeodomain (PHD) domain that can specifically bind to the unmethylated H3 tail terminus 13, 14, 15. This biochemical property has led to an idea that histone tails in chromatin might provide a docking site for region-specific methylation by the de novo methyltransferases 14, 15. We recently found that the histone H3 tail is indeed essential for DNA methylation by providing a direct contact with Dnmt3a-Dnmt3L 16. However, the exact mechanism for the transformation of a chromatin modification status into DNA methylation is left unexplored.
The current model of DNA methylation 7, 17 underscores the recruitment of Dnmt3 enzymes to genomic targets via direct or indirect binding to histone tails 13, 14, 15, 18. While this model assumes that the histone tail with a specific modification state specifies a docking site for Dnmts as for many other chromatin binding/modifying proteins, it does not consider the possibility that the enzymatic activity of Dnmts might be regulated by histone tails and their modification. In this study, we explore the role of the histone tail-Dnmt3a interaction and provide evidence for allosteric regulation of Dnmt3a by histone tails in DNA methylation in mammalian cells.
Results
The enzymatic activity of Dnmt3a is stimulated in vitro by histone H3 peptides lacking lysine methylation
The PHD domain of Dnmt3a is able to bind histone tails in vitro 14, 15, but is dispensable for the enzyme association with chromatin in vivo 19, 20. These observations suggest that recruitment of Dnmt3a to chromatin could be independent of the PHD domain-mediated binding to the H3 N-terminus. On the other hand, the PHD domain of Dnmt3a interacts with the catalytic domain as shown in both GST pull-down and yeast two-hybrid assays (Supplementary information, Figure S1), suggesting that binding of histone tails might play a regulatory role for the catalytic activity of Dnmt3a. To examine this possibility, recombinant Dnmt3a purified from transiently transfected 293T cells was used for in vitro methylation assay in the presence of histone peptides. Strikingly, the activity of Dnmt3a was stimulated by H3 peptides (amino acid 1-21) depending on the methylation status of lysine 4 (K4) (Figure 1A). While trimethylated peptides had little effect, di-, mono- and unmethylated peptides increased the Dnmt3a methylation activity by 2-, 4-, to 8-fold respectively. To explore the mechanism by which H3 peptides stimulate Dnmt3a activity, we measured DNA methlyation rates at different concentrations of S-adenosyl-methionine (SAM) and DNA substrates. In the presence of H3, the maximal observed rate increased by 10.1-fold during titration of SAM (Figure 1B). A similar result was observed with titration of the DNA substrate (Figure 1C). Notably, the SAM concentration required to reach the half-maximal reaction rate was almost unchanged while this value increased by 7.2-fold for the DNA substrate. We also measured the dissociation constant (Kd) of DNA binding of Dnmt3a by SPR assay. The presence of H3 peptides did not lead to any change in the binding curve and thus binding affinity was unchanged (Supplementary information, Figure S2). These results suggest that H3 peptides stimulate the methylation reaction by Dnmt3a mainly through increasing the turnover rate.
The binding ability to histone tails is essential for Dnmt3a to be stimulated in vitro and to methylate DNA in vivo
We next examined whether binding of histone H3 peptides to the PHD domain is involved in stimulation of Dnmt3a enzymatic activity by free H3 tail. Two residues at the H3 recognition interface in the PHD structure 14 were therefore chosen for mutational analysis. Asp308, which forms part of the H3 K4-binding pocket via its carboxylate, was substituted by alanine (A) to disrupt the specific interaction. Met325, important for the recognition of H3 N-terminal residues, was replaced by bulky tryptophan (W) to create steric hindrance. As expected, the PHD domains bearing these mutations lost binding affinity for histone peptides (Figure 2A). The full-length Dnmt3a proteins bearing these mutations (Supplementary information, Figure S3) were also found to lack H3 binding ability in peptide pull-down assay (Supplementary information, Figure S4A). Unlike the catalytically inactive mutant E533A that lacked any enzymatic activity, the basal methylation activity of these two PHD mutants was similar to that of the wild-type Dnmt3a. However, these two mutants were no longer able to respond to the H3 peptide stimulation as the wild type (Figure 2B). These results suggest that the allosteric activation of Dnmt3a by H3 peptides is mediated by their binding to the PHD domain.
To evaluate the role of H3 binding by Dnmt3a for DNA methylation in vivo, we performed an episomal plasmid methylation assay in transfected human cells 21. Southern hybridization analysis showed that a significant portion of the episomal DNA became methylated when co-transfected with the wild-type Dnmt3a (Figure 2C). However, the three mutants affecting the PHD domain showed no or little methylation activity. A more biologically relevant functional assay was also conducted by introducing mutant proteins into mouse ES cells deficient in Dnmt3a and Dnmt3b 22. Stable cell lines ectopically expressing wild-type and mutant Dnmt3a were established (Supplementary information, Figure S4B). Chromatin IP (ChIP) analysis demonstrated binding of the two D308A and M325W mutant proteins to the pluripotency gene Oct4 promoter in ES cells (Supplementary information, Figure S4C). However, both mutants failed to methylate the promoter region of Oct4 during its silencing (Figure 2D and Supplementary information, Figure S4D). These data suggest that allosteric activation by H3 binding might be required for the in vivo function of Dnmt3a.
Characterization of mutations in Dnmt3a that abrogate its catalytic activation by histone peptides but not by the H3 binding and the basal methylation activity
As previous studies indicate that the Dnmt3a PHD domain is not required for its chromatin association 19, 20, we reasoned that the binding of H3 peptide via the PHD domain might regulate the Dnmt3a function through allosteric enzymatic activation rather than chromatin recruitment. As the allosteric effect of H3 binding might be mediated by interaction between the PHD and catalytic domains, we sought to characterize crucial residues at the interaction interfaces, whose substitutions would block allosteric activation of enzymatic activity but have no effect on the H3 binding and the basal catalytic activities. Based on the structures of PHD 14 and C-terminal catalytic domain 23 of Dnmt3a, and the structure of the full-length Dnmt3L 13, which covers both the PHD and C-terminal part, we modeled the potential interfaces by homology docking (Figure 3A). Residues on both interfaces were chosen for mutational analysis. Among 13 residues we analyzed (Supplementary information, Table S1), alanine substitution of glutamine 304 (Q304) in the PHD domain severely reduced the degree of activation of Dnmt3a by histone peptides (Figure 3B), yet the histone peptide-binding property of the mutant protein remained unchanged (Figure 3C and 3D). Similarly, alanine substitution of arginine 580 (R580) in the catalytic domain rendered the enzyme insensitive to the stimulation by H3 peptides, while the basal methylation activity in the absence of H3 peptides (Figure 3B) and histone binding ability (Figure 3C) did not change. These data demonstrate the presence of interfaces involved in allosteric regulation that is independent of the histone tail binding and basal catalytic activities.
Mutations affecting allosteric activation by histone H3 do not affect genomic binding of Dnmt3a but diminish its methylation function in ES cells
Similar to the PHD mutations, the two interface mutations Q304A and R580A diminished the methylation activity of Dnmt3a when expressed in cultured human cells (Supplementary information, Figure S5). We next determined the physiological importance of allosteric regulation of Dnmt3a by examining the impact of interface mutations on DNA methylation in ES cells. Stable cell lines expressing exogenous Dnmt3a at similar levels (Supplementary information, Figure S6A) were established from triple knockout (TKO) ES cells deficient in Dnmt1, Dnmt3a and Dnmt3b 24. Cellular fractionation assay (Supplementary information, Data S1) confirmed that the mutant and wild-type proteins were similarly detected in the S1 and S2 fractions rich in euchromatin and heterochromatin, respectively (Supplementary information, Figure S6B). Immunoprecipitation assay showed that the Dnmt3a proteins were associated with Dnmt3L (Figure 4A), consistent with the previous finding 13. To examine whether the point mutation would change genomic localization of the Dnmt3a protein, we performed ChIP for two types of the known target sequences. Quantification by real-time PCR of co-immunoprecipitated DNA indicated that mutant proteins were enriched on the major satellite repeats (Figure 4B). Similarly, ChIP analysis revealed binding of the two mutant proteins to the Oct4 promoter upon induction of cell differentiation, concomitant with H3K4 demethylation accompanying gene silencing (Figure 4C and 4D). These observations suggest that the two interface mutants have unaltered interaction with other proteins and chromatin in vivo.
We next examined the function of the ectopic Dnmt3a in genome-wide methylation. Quantification of the methylcytosine content by HPLC indicated that expression of wild-type Dnmt3a in two independent cell lines could restore the methylation level to 0.8% and 1.4% while expression of the two mutant proteins resulted in no or much less methylation (Figure 5A). Analysis of specific target sequences extended this observation. As shown by Southern blotting analysis, major satellite repeats became substantially methylated upon ectopic expression of the wild-type Dnmt3a, while they were nearly unmethylated in cells expressing the Dnmt3a mutants, much like in the original TKO cells (Figure 5B). Similarly, both mutants were unable to methylate the promoter regions of the single-copy genes Oct4 and Sox30 during ES cell differentiation (Figure 5C and Supplementary information, Figure S7), while the wild-type Dnmt3a showed robust methylation. Therefore, point mutations that affect interfaces of both the PHD and catalytic domains and thus abrogate the allosteric effect of histones led to loss of the methylation function in vivo. As association of both mutant proteins with chromatin and their genomic targets did not appear to be affected, the failure in DNA methylation can be attributed to defective allosteric activation of Dnmt3a by histone tails in vivo in the chromatin context. Interestingly, one of the missense mutations, R803S, recently identified in human DNMT3A of acute myeloid leukemia patients 3, corresponds to the interface mutation R580A in sequence alignment. Thus, defective allosteric activation by histones can be implicated in the malfunction of mutant DNMT3A in human leukemogenesis.
Discussion
The data described in this and other previous work lead us to propose that occurrence of DNA methylation might take two steps – enzyme recruitment and activation (Figure 5D). In this model, the methyltransferase is recruited to a specific genomic site by directly recognizing nucleosomal DNA or histones 13, 14, 15, 18, 25, or alternatively through other adaptor proteins such as Dnmt3L 13, 23, G9a 26, 27, 28, Np95 29 and PML-RAR 30. The localized Dnmt3 is then allosterically activated to methylate nearby cytosines upon the binding of an unmodified H3K4 N-terminus to the PHD domain. Other sequences are excluded from methylation either because they are not loaded with Dnmts or their associated chromatin is not permissive due to K4 methylation. In agreement with our working model, epigenomic studies reveal that DNA cytosine methylation has no simple linear relationship with histone modification, except for the anti-correlation with H3K4 trimethylation 11, 12, 31. Requirement of allosteric activation by histone tails for Dnmt3a function might also account for the enrichment of DNA methylation in nucleosome-associated exons reported most recently 32.
The dual requirement for recruitment and activation provides an opportunity at the chromatin level to converge multiple cellular signals into long-term silencing of target genes. Presumably, there might be an advantage for patterns of histone modification that are not propagated efficiently during mitosis to be converted into patterns of DNA methylation that can be stably maintained. This is particularly relevant in case of pluripotency genes such as Oct4 when this vitally important regulatory gene undergoes multi-step down-regulation during cell lineage commitment in response to intrinsic and extrinsic signals in early embryos 33, 34. The repression process involves binding of transcription factor GCNF 34 and H3K9 methyltransferase G9a 28 to the Oct4 promoter, both of which are necessary events for DNA methylation to ensue. G9a mediates the recruitment of Dnmt3 enzymes independent of its catalytic activity 28, arguing against the proposed role of H3K9 methylation as a signal for DNA methylation 33. In agreement with this, histone tails required to activate the Dnmt3a only include a few N-terminal amino acids and the portion including K9 can even be deleted without reducing cytosine methylation by ectopic expressed Dnmt3a-Dnmt3L in yeast 16. Moreover, inclusion of K9 trimethylation in the free H3 peptides (this work) or in the H3 tail of reconstituted chromatin 15 has little effect on the in vitro methylation activity. It is therefore unlikely for the DNA methyltransferase to respond directly to the change in H3K9 methylation. Given the implication of allosteric activation by H3 tails with lower methylation levels at K4, H3K4 demethylation instead appears to be a major checkpoint for DNA methylation. Consistent with this idea, mutations blocking the allosteric activation at the intra-molecule interfaces in Dnmt3a diminished its methylation activity in ES cells, but they did not change its complex formation with Dnmt3L, overall association with chromatin and the recruitment to major satellite repeats and the Oct4 promoter. Thus, mere physical recruitment of the DNA methyltransferase to targets, while required, would not necessarily trigger DNA methylation. Histone modifications, especially H3K4 methylation, regulate the allosteric activation of recruited DNA methyltransferase. Further investigation is needed to reveal the mechanism underlying the coordinate actions of the factors that target Dnmts to chromatin, modify chromatin and trigger DNA methylation.
Materials and Methods
Protein purification
For purification of Flag-tagged full-length Dnmt3a, HEK293T cells were transiently transfected with expression constructs encoding Flag-tagged Dnmt3a (the ES form, amino acid 1-689) or its mutants by calcium phosphate transfection method. Cells were harvested 48 h post transfection and lysed in high salt lysis buffer (50 mM Tris-Cl, pH 7.5, 500 mM NaCl, 1% Triton X-100, 1 mM EDTA and protease inhibitor cocktail (Roche)). After centrifugation at 100 000× g for 60 min at 4 °C, the clarified cell lysate was collected and incubated with FLAG-M2 agarose (Sigma) by gently rotating at 4 °C for 2 h. Following washing with lysis buffer three times, bound proteins were eluted by competition with FLAG peptides and dialyzed into storage buffer (10 mM HEPES-KOH, pH 7.5, 50 mM KCl, 1 mM EDTA and 50% glycerol).
For purification of GST fusion proteins, GST-tagged Dnmt3aPHD was overexpressed in E. coli strain BL21 (DE3) CodonPlus-RIL (Stratagene). Cells were grown at 37 °C in LB medium complemented with 100 μM zinc sulfate to an optical density of 0.6-0.8 at 600 nm, and then induced with 0.2 mM IPTG at 20 °C overnight. Purification was performed using Glutothione Sepharose 4B (GE healthcare) according to the manufacturer's instructions.
In vitro methylation assay
In vitro methylation activity of Dnmt3a was determined as described 35 with slight modifications. In brief, purified Flag-Dnmt3a protein (200 ng, 0.13 μM) was pre-incubated with or without H3 peptides (100 ng, 2.3 μM) in 20 mM HEPES, pH 7.5, 50 mM KCl, 1 mM EDTA, 50 μg/ml BSA and 1.25 mM 3H-SAM (80 Ci/mmol, Perkin Elmer) in a total volume of 19 μl at room temperature for 5 min. Biotinylated 2.2-kb DNA fragment (100 ng) (0.34 μM CpGs) amplified from the EBNA1 region of p220.2 was added to the reaction and incubated for 20 min at 37 °C. Reactions were quenched by addition of excess of cold SAM. Incorporation of methyl-3H into the naked substrate DNA was determined as described 36.
In vivo methylation assay
In vivo activity of Dnmt3a was mainly analyzed in stably transfected ES cell lines. Methylcytosine content in genomic DNA was determined by HPLC as described 37. Methylation level in major satellite repeats was assessed by Southern hybridization using non-radioactive method with biotin-labeled oligonucleotide (5′-biotin-TATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAG-3′). Methylation status at gene promoters was determined by bisulfite sequencing analysis as described 38. The in vivo activity of Dnmt3a toward episomal plasmid in HEK293 c18 cells was analyzed essentially as described 21.
Establishment of stable ES cell lines and in vitro differentiation
DKO (Dnmt3a and Dnmt3b double knockout 39) stable ES cells ectopically expressing Dnmt3a were established essentially as described 20. TKO (Dnmt31, Dnmt3a, Dnmt3b TKO 24) stable ES cells lines ectopically expressing Dnmt3a were established by infection with lentivirus carrying a Dnmt3a expression cassette linked to the EGFP gene. ES clones expressing EGFP were picked and expanded. Positive clones expressing FLAG-HA-Dnmt3a were verified by western blotting. ES cell in vitro differentiation was performed as described 34.
Histone peptide binding assay
Histone peptide pull-down assays were performed as described 40. Biotinylated histone peptides of high purity were synthesized by Scilight-Peptide Inc. Briefly, 1 μg of peptides were incubated with 1 μg of purified protein in binding buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1% NP-40 and 1 mM PMSF) overnight at 4 °C. A volume of 20 μl of streptavidin beads (GE healthcare) were added into the mixture and incubated for another 2 h. After washing three times with washing buffer (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 0.1% NP-40 and 1 mM PMSF), the bound proteins were eluted in 2× SDS loading buffer by heating at 100 °C for 5 min and subjected to western analysis.
Surface plasmon resonance measurements
Surface plasmon resonance binding experiments were performed on BIAcore 3000 biosensor at 25 °C as described previously 41. Equivalent amount of H3 (1-21) peptides biotin-labeled at the C-terminus were immobilized in four different cells of one streptavidin-coated biosensor chip. The immobilization response unit of peptides containing unmethylated, mono-, di- and tri-methylated K4 was about 150. The wild-type and mutant GST-Dnmt3a PHD proteins were dialyzed in running buffer containing 10 mM HEPES, pH 7.5, 150 mM NaCl and 0.005% NP-20 at the concentration of 50 μM. A 3-min sample injection was performed under the flow rate of 30 μl/min and the disassociation time was 15 min. Chip was regenerated by washing with 10 mM NaOH. All measurements were repeated at least three times under the same condition.
Chromatin immunoprecipitation
The procedures are carried out mainly as previously described 42. Briefly, the crosslinked chromatin of ES cells or embryonic body was extracted and sheared to average DNA size of 500 bp with Diagenode BioRuptor™ sonicator. The sheared extracts were diluted 10-fold by ChIP dilution buffer and precleared with 30 μl of protein A/G agarose beads. Following incubation with antibodies (anti-H3K4me3, ab8480; anti-H3, ab1791; anti-Dnmt3a, self-parepared) at 4 °C overnight, 30 μl of protein A/G agarose beads blocked with BSA and salmon sperm DNA were added and rotated for 2 h to capture the protein-DNA complex. After washing with a series of wash buffer, the bound protein-DNA complex was eluted with 100 mM NaHCO3 and 1% SDS. After crosslink reversal and proteinase K treatment, the purified DNA was subjected to real-time PCR to determine the relative enrichment fold compared with IgG. Primers for Oct4 promoter are as follows, Oct4_ChIP_f: 5′-TGGGGCATCCGAGCAACTGGT-3′ and Oct4_ChIP_r: 5′-AGAGCTGTGGGGGTGGAGAAACTGA-3′.
References
Jaenisch R, Bird A . Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals. Nat Genet 2003; 33 Suppl:245–254.
Bestor TH . The DNA methyltransferases of mammals. Hum Mol Genet 2000; 9:2395–2402.
Ley TJ, Ding L, Walter MJ, et al. DNMT3A mutations in acute myeloid leukemia. N Engl J Med 2010; 363:2424–2433.
Ciccone DN, Su H, Hevi S, et al. KDM1B is a histone H3K4 demethylase required to establish maternal genomic imprints. Nature 2009; 461:415–418.
Dennis K, Fan T, Geiman T, Yan Q, Muegge K . Lsh, a member of the SNF2 family, is required for genome-wide methylation. Genes Dev 2001; 15:2940–2944.
Fan Y, Nikitina T, Zhao J, et al. Histone H1 depletion in mammals alters global chromatin structure but causes specific changes in gene regulation. Cell 2005; 123:1199–1212.
Cheng X, Blumenthal RM . Coordinated chromatin control: structural and functional linkage of DNA and histone methylation. Biochemistry 2010; 49:2999–3008.
Li E . Chromatin modification and epigenetic reprogramming in mammalian development. Nat Rev Genet 2002; 3:662–673.
Tamaru H, Selker EU . A histone H3 methyltransferase controls DNA methylation in Neurospora crassa. Nature 2001; 414:277–283.
Lehnertz B, Ueda Y, Derijck AA, et al. Suv39h-mediated histone H3 lysine 9 methylation directs DNA methylation to major satellite repeats at pericentric heterochromatin. Curr Biol 2003; 13:1192–1200.
Mohn F, Weber M, Rebhan M, et al. Lineage-specific polycomb targets and de novo DNA methylation define restriction and potential of neuronal progenitors. Mol Cell 2008; 30:755–766.
Meissner A, Mikkelsen TS, Gu H, et al. Genome-scale DNA methylation maps of pluripotent and differentiated cells. Nature 2008; 454:766–770.
Ooi SK, Qiu C, Bernstein E, et al. DNMT3L connects unmethylated lysine 4 of histone H3 to de novo methylation of DNA. Nature 2007; 448:714–717.
Otani J, Nankumo T, Arita K, et al. Structural basis for recognition of H3K4 methylation status by the DNA methyltransferase 3A ATRX-DNMT3-DNMT3L domain. EMBO Rep 2009; 10:1235–1241.
Zhang Y, Jurkowska R, Soeroes S, et al. Chromatin methylation activity of Dnmt3a and Dnmt3a/3L is guided by interaction of the ADD domain with the histone H3 tail. Nucleic Acids Res 2010; 38:4246–4253.
Hu JL, Zhou BO, Zhang RR, et al. The N-terminus of histone H3 is required for de novo DNA methylation in chromatin. Proc Natl Acad Sci USA 2009; 106:22187–22192.
Cedar H, Bergman Y . Linking DNA methylation and histone modification: patterns and paradigms. Nat Rev Genet 2009; 10:295–304.
Dhayalan A, Rajavelu A, Rathert P, et al. The Dnmt3a PWWP domain reads histone 3 lysine 36 trimethylation and guides DNA methylation. J Biol Chem 2010; 285:26114–26120.
Ge YZ, Pu MT, Gowher H, et al. Chromatin targeting of de novo DNA methyltransferases by the PWWP domain. J Biol Chem 2004; 279:25447–25454.
Chen T, Tsujimoto N, Li E . The PWWP domain of Dnmt3a and Dnmt3b is required for directing DNA methylation to the major satellite repeats at pericentric heterochromatin. Mol Cell Biol 2004; 24:9048–9058.
Hsieh CL . In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b. Mol Cell Biol 1999; 19:8211–8218.
Chen T, Ueda Y, Dodge JE, Wang Z, Li E . Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b. Mol Cell Biol 2003; 23:5594–5605.
Jia D, Jurkowska RZ, Zhang X, Jeltsch A, Cheng X . Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation. Nature 2007; 449:248–251.
Tsumura A, Hayakawa T, Kumaki Y, et al. Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b. Genes Cells 2006; 11:805–814.
Jones PA, Liang G . Rethinking how DNA methylation patterns are maintained. Nat Rev Genet 2009; 10:805–811.
Dong KB, Maksakova IA, Mohn F, et al. DNA methylation in ES cells requires the lysine methyltransferase G9a but not its catalytic activity. EMBO J 2008; 27:2691–2701.
Tachibana M, Matsumura Y, Fukuda M, Kimura H, Shinkai Y . G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription. EMBO J 2008; 27:2681–2690.
Epsztejn-Litman S, Feldman N, Abu-Remaileh M, et al. De novo DNA methylation promoted by G9a prevents reprogramming of embryonically silenced genes. Nat Struct Mol Biol 2008; 15:1176–1183.
Meilinger D, Fellinger K, Bultmann S, et al. Np95 interacts with de novo DNA methyltransferases, Dnmt3a and Dnmt3b, and mediates epigenetic silencing of the viral CMV promoter in embryonic stem cells. EMBO Rep 2009; 10:1259–1264.
Di Croce L, Raker VA, Corsaro M et al. Methyltransferase recruitment and DNA hypermethylation of target promoters by an oncogenic transcription factor. Science 2002; 295:1079–1082.
Weber M, Hellmann I, Stadler MB, et al. Distribution, silencing potential and evolutionary impact of promoter DNA methylation in the human genome. Nat Genet 2007; 39:457–466.
Chodavarapu RK, Feng S, Bernatavichute YV, et al. Relationship between nucleosome positioning and DNA methylation. Nature 2010; 466:388–392.
Feldman N, Gerson A, Fang J, et al. G9a-mediated irreversible epigenetic inactivation of Oct-3/4 during early embryogenesis. Nat Cell Biol 2006; 8:188–194.
Fuhrmann G, Chung AC, Jackson KJ, et al. Mouse germline restriction of Oct4 expression by germ cell nuclear factor. Dev Cell 2001; 1:377–387.
Xie ZH, Huang YN, Chen ZX, et al. Mutations in DNA methyltransferase DNMT3B in ICF syndrome affect its regulation by DNMT3L. Hum Mol Genet 2006; 15:1375–1385.
Roth M, Jeltsch A . Biotin-avidin microplate assay for the quantitative analysis of enzymatic methylation of DNA by DNA methyltransferases. Biol Chem 2000; 381:269–272.
Ramsahoye BH . Measurement of genome wide DNA methylation by reversed-phase high-performance liquid chromatography. Methods 2002; 27:156–161.
Li JY, Pu MT, Hirasawa R, et al. Synergistic function of DNA methyltransferases Dnmt3a and Dnmt3b in the methylation of Oct4 and Nanog. Mol Cell Biol 2007; 27:8748–8759.
Okano M, Bell DW, Haber DA, Li E . DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 1999; 99:247–257.
Shi X, Hong T, Walter KL, et al. ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression. Nature 2006; 442:96–99.
Li H, Ilin S, Wang W, et al. Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF. Nature 2006; 442:91–95.
Hu YG, Hirasawa R, Hu JL, et al. Regulation of DNA methylation activity through Dnmt3L promoter methylation by Dnmt3 enzymes in embryonic development. Hum Mol Genet 2008; 17:2654–2664.
Acknowledgements
We thank Gerd Pfeifer (Beckman Research Institute of City of Hope) and Taiping Chen (Novartis Institutes for Biomedical Research) for helpful discussions and Jiemin Wong (East China Normal University) for critical reading of the manuscript. We are grateful to Masaki Okano (RIKEN Center for Developmental Biology) for providing Dnmt triple knockout ES cells. This work was supported by grants from the Ministry of Science and Technology of China (2005CB522400 and 2006CB943900), the National Natural Science Foundation of China (30730059) and the Shanghai Municipal Government (08dj1400501, 0852S11223) to GLX.
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Supplementary information
Supplementary information, Figure S1
The Dnmt3a PHD domain interacts with the catalytic domain. (PDF 83 kb)
Supplementary information, Figure S2
Measurement of binding affinity of Flag-Dnmt3a toward DNA. (PDF 98 kb)
Supplementary information, Figure S3
Coomassie staining of Flag-tagged full-length Dnmt3a proteins purified from transiently transfected HEK293T cells and GST-fusion proteins of 3aPHD purified from E. coli BL21 (DE3). (PDF 169 kb)
Supplementary information, Figure S4
Functional analysis of Dnmt3a D308A and M325W mutants. (PDF 146 kb)
Supplementary information, Figure S5
Southern hybridization analysis of the methylation activity of transiently transfected Dnmt3a interface mutants. (PDF 121 kb)
Supplementary information, Figure S6
Expression and distribution of Dnmt3a Q304A and R580A mutants in mouse ES cells. (PDF 160 kb)
Supplementary information, Figure S7
Methylation analysis at the Oct4 and Sox30 promoters in ES cells ectopically expressing wild-type and mutant Dnmt3a. (PDF 146 kb)
Supplementary Table 1.
Mutational analysis of residues on the interfaces of the PHD and catalytic domains of Dnmt3a. (PDF 90 kb)
Supplementary information, Data S1
Materials and Methods (PDF 61 kb)
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Li, BZ., Huang, Z., Cui, QY. et al. Histone tails regulate DNA methylation by allosterically activating de novo methyltransferase. Cell Res 21, 1172–1181 (2011). https://doi.org/10.1038/cr.2011.92
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DOI: https://doi.org/10.1038/cr.2011.92
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