Abstract
INDIRECT evidence has implicated an interaction between the cytoskeleton and the protein synthetic machinery1–8. Two recent reports have linked the elongation factor la (EF-la) which is involved in protein synthesis, with the microtubular cytoskeleton7–8.In situ hybridization has, however, revealed that the messages for certain cytoskeletal proteins are preferentially associated with actin filaments6. ABP-50 is an abundant actin filament bundling protein of native relative molecular mass 50,000 (50K)9 isolated from Dictyostelium discoideum. Immunofluorescence studies show that ABP-50 is present in filopodia and other cortical regions that contain actin filament bundles9,10. In addition, ABP-50 binds to monomeric actin in the cytosol of unstimulated cells and the association of ABP-50 with the actin cytoskeleton is regulated during chemotaxis10. Through complementary DNA sequencing and subsequent functional analysis, we have identified ABP-50 as D. discoideum EF-la. The ability of EF-la to bind reversibly to the actin cytoskeleton upon stimulation could provide a mechanism for spatially and temporally regulated protein synthesis in eukaryotic cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Lenk, R., Ransom, L., Kaufmann, Y. & Penman, S. Cell 10, 67–78 (1977).
Nielsen, P., Goelz, S. & Trachsel, H. Cell Biol. Int. Rept. 7, 245–254 (1983).
Bonneau, A., Darveau, A. & Sonenberg, N. J. Cell Biol. 100, 1209–1218 (1985).
Ornelles, D. A., Fey, E. G. & Penman, S. Molec. cell. Biol. 6, 1650–1662 (1986).
Cheng, H. & Bjerknes, M. J. molec. Biol. 210, 541–549 (1989).
Singer, R. H., Langevin, G. L. & Lawrence, J. B. J. Cell Biol. 108, 2343–2353 (1989).
Kuriyama, R., Savereide, P., Lefebvre, P. & Dasgupta, S. J. Cell Sci. 95, 231–236 (1990).
Ohta, K. et al. J. biol. Chem. 265, 3240–3247 (1990).
Demma, M., Warren, V., Hock, R., Dharmawardhane, S. & Condeelis, J. J. biol. Chem. 265, 2286–2291 (1990).
Dharmawardhane, S., Demma, M., Warren, V. & Condeelis, J. J. Cell Biol. 109, 273a (1989).
Uetsuki, T., Naito, A., Nagata, S. & Kaziro, Y. J. biol. Chem. 264, 5791–5798 (1989).
Pokalsky, A. R. et al. Nucleic Acids Res. 17, 4661–4673 (1989).
van Hemert, F. J., Amons, R., Pluijms, W. J. M., van Ormondt, H. & Möller, W. EMBO J. 3, 1109–1113 (1984).
Nagata, S. et al. EMBO J. 3, 1825–1830 (1984).
Linz, J. E., Lira, L. M. & Sypherd, P. S. J. biol. Chem. 261, 15022–15029 (1986).
Tabin, C. J. et al. Nature 300, 143–149 (1982).
Jurnak, F. Science 230, 32–36 (1985).
Taparowsky, E., Shimizu, K., Goldfarb, M. & Wigler, M. Cell 34, 581–586 (1983).
Bosch, L. et al. Trends biochem. Sci. 10, 313–316 (1985).
Sutoh, K. & Mabuchi, I. Biochemistry 28, 102–106 (1989).
Merrick, W. C. Meth. Enzym. (eds Moldave, K. & Grossman, L.) 60, 108–123 (Academic, New York, 1979).
Davis, L. G., Dibner, M. D. & Battey, J. F. Basic Methods in Molecular Biology (Elsevier, New York, 1986).
Woolley, P. & Clark, B. F. C. Bio/technology 7, 913–920 (1989).
Franke, J., Podgorski, G. J. & Kessin, F. H. Devl Biol. 124, 504–511 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yang, F., Demma, M., Warren, V. et al. Identification of an actin-binding protein from Dictyostelium as elongation factor 1a. Nature 347, 494–496 (1990). https://doi.org/10.1038/347494a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/347494a0
This article is cited by
-
Tyrosyl-tRNA synthetase has a noncanonical function in actin bundling
Nature Communications (2023)
-
Early defects in translation elongation factor 1α levels at excitatory synapses in α-synucleinopathy
Acta Neuropathologica (2019)
-
The eukaryotic translation elongation factor 1A regulation of actin stress fibers is important for infectious RSV production
Virology Journal (2018)
-
Heterozygous mutation of eEF1A1b resulted in spermatogenesis arrest and infertility in male tilapia, Oreochromis niloticus
Scientific Reports (2017)
-
Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast
Nature Communications (2016)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
