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7q deletion mapping and expression profiling in uterine fibroids

Oncogene volume 24, pages 6545–6554 (2005)Cite this article

Abstract

Uterine fibroids are some of the most common tumours of females, but relatively little is known about their molecular basis. Several studies have suggested that deletions on chromosome 7q could have a role in fibroid formation. We analysed 165 sporadic uterine fibroids to define a small 3.2 megabase (Mb) commonly deleted region on 7q22.3–q31.1, flanked by clones AC005070 and AC007567. We also used oligonucleotide microarrays to compare the expression profiles of 10 samples of normal myometrium and 15 fibroids, nine of which displayed 7q-deletions. Activating transcription factor 3, patched homolog (Drosophila), homeo box A5, death-associated protein kinase 1, and retinoic acid receptor responder 3 were downregulated, and excision repair crosscomplementing 3, transcription factor AP-2 gamma and protein kinase C beta 1 were upregulated in fibroids. New pathways were discovered related to fibroid formation. The presence or absence of 7q-deletions did not dramatically affect the global expression pattern of the tumours; changes, however, were observed in genes related to vesicular transport and nucleic acid binding.

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Acknowledgements

We thank Sini Marttinen for helping with the samples and the Finnish Cancer Society, the Helsinki University Central Hospital, the Sigrid Juselius Foundation, the Maud Kuistila Foundation, The Finnish Medical Foundation and the Academy of Finland (Grant 44870, Finnish Center of Excellence Program 2000–2005) for financial support.

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Authors and Affiliations

  1. Department of Medical Genetics, University of Helsinki,

    Sakari Vanharanta, Päivi Laiho, Auli Karhu, Diego Arango & Lauri A Aaltonen

  2. Biomedicum Helsinki, FIN-00014, Finland

    Sakari Vanharanta, Päivi Laiho, Auli Karhu, Diego Arango & Lauri A Aaltonen

  3. Molecular and Population Genetics Laboratory, Cancer Research UK, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK

    Noel C Wortham & Ian P Tomlinson

  4. Department of Obstetrics and Gynaecology, Helsinki University Central Hospital, PO Box 22, Haartmaninkatu 2, FIN-00029, Finland

    Jari Sjöberg

  5. Department of Clinical Genetics, Helsinki University Central Hospital, PO Box 140, Haartmaninkatu 2 B, FIN-00029, Finland

    Kristiina Aittomäki

  6. Department of Pathology, University of Helsinki, PO Box 21, Haartmaninkatu 3, FIN-00014, Finland

    Johanna Arola

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  1. Sakari Vanharanta
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Correspondence to Lauri A Aaltonen.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc)

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Vanharanta, S., Wortham, N., Laiho, P. et al. 7q deletion mapping and expression profiling in uterine fibroids. Oncogene 24, 6545–6554 (2005). https://doi.org/10.1038/sj.onc.1208784

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  • DOI: https://doi.org/10.1038/sj.onc.1208784

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