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Three-dimensional structure of bovine cytochrome bC1 complex by electron cryomicroscopy and helical image reconstruction

Nature Structural Biology volume 3, pages 553–561 (1996)Cite this article

Abstract

Cytochrome bc1 complex from bovine heart has been reconstituted into tubular crystals. The three-dimensional structure of the complex in lipid bilayer has been obtained at an effective resolution of 16 Å by electron cryomicroscopy and helical image reconstruction. The complex is in a dimeric form, in which the monomers are associated closely in extramembrane domains on both sides of the membrane. The large inner domain is distinctively hollow and the small outer domain consists of a flat mass and two bulbous extrusions. These domains are connected by two narrow transmembrane columns. Locations of the subunits and the redox centres in the model are proposed.

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Authors and Affiliations

  1. 1nternational Institute for Advanced Research, Matsushita Electric Industrial Co., Ltd., 3–4 Hikaridai, Seika, Kyoto, 619-02, Japan

    Toshihiko Akiba & Keiichi Namba

  2. Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan

    Chikashi Toyoshima

  3. Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka, 560, Japan

    Takateru Matsunaga, Masahide Kawamoto & Keiichi Fukuyama

  4. National Institute of Biosdence and Human-Technology, Tsukuba, Ibaraki, 305, Japan

    Tomomi Kubota

  5. Department of Biochemistry, Faculty of Science, Okayama University of Science, Okayama, Okayama, 700, Japan

    Hiroshi Matsubara

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Akiba, T., Toyoshima, C., Matsunaga, T. et al. Three-dimensional structure of bovine cytochrome bC1 complex by electron cryomicroscopy and helical image reconstruction. Nat Struct Mol Biol 3, 553–561 (1996). https://doi.org/10.1038/nsb0696-553

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