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Release of Neurohypophysial Hormones by Exocytosis

Nature New Biology volume 241, pages 86–88 (1973)Cite this article

Abstract

ELECTRON microscopy has provided evidence for release of neurohypophysial hormones by exocytosis (emiocytosis)1–3. If this concept is correct, the soluble content of the neurosecretory granules (that is, the peptide hormones and their carrier-proteins, the neurophysins) should be released4–7 without coming into contact with the cytosol during the process of extrusion and without the occurrence of a generalized increase in the permeability of the cell membrane to peptides and proteins. We have therefore determined whether, in isolated neurohypophyses, cytoplasmic constituents escape along with the hormones and neurophysins into the incubation medium by using the leakage of lactic dehydrogenase (LDH), a cytoplasmic enzyme, to check the selectivity of the secretory process8.

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References

  1. Nagasawa, J., Douglas, W. W., and Schulz, R., Nature, 227, 407 (1970).

    Article  CAS  Google Scholar 

  2. Douglas, W. W., Nagasawa, J., and Schulz, R., Mem. Soc. Endocrinol., 19, 353 (1971).

    Google Scholar 

  3. Krisch, B., Becker, K., and Bargmann, W. Z., Zellforsch. Mikrosk. Anat., 123, 47 (1972).

    Article  CAS  Google Scholar 

  4. Fawcett, C. P., Powell, A. E., and Sachs, J., Endocrinology, 83, 1299 (1968).

    Article  CAS  Google Scholar 

  5. Uttenthal, L. O., Livett, B. G., and Hope, D. B., Phil. Trans. Roy. Soc. B., 261, 379 (1971).

    Article  CAS  Google Scholar 

  6. Burton, A. M., Forsling, M. L., and Martin, M. J., J. Physiol., 217, 23P (1971).

    Article  CAS  Google Scholar 

  7. Nordmann, J. J., Dreifuss, J. J., and Legros, J. J., Experientia, 27, 1344 (1971).

    Article  CAS  Google Scholar 

  8. Schneider, F. H., Smith, A. D., and Winkler, H., Brit. J. Pharmac. Chemother., 31, 94 (1967).

    Article  CAS  Google Scholar 

  9. Weiss, C. B., and Goodman, F. R., J. Pharmac. Exp. Ther., 169, 46 (1969).

    CAS  Google Scholar 

  10. Van Breemen, C., and De Weer, P., Nature, 226, 760 (1970).

    Article  CAS  Google Scholar 

  11. De Bassio, W. A., Schnitzler, R. M., and Parsons, R. L., J. Neurobiol., 2, 263 (1971).

    Article  CAS  Google Scholar 

  12. Heuser, J., and Miledi, R., Proc. Roy. Soc. B., 179, 247 (1971).

    CAS  Google Scholar 

  13. Kajimoto, N., and Kirkepar, S. M., Nature, 235, 29 (1972).

    CAS  Google Scholar 

  14. Dreifuss, J. J., Grau, J. D., and Bianchi, R. E., Experientia, 27, 1295 (1971).

    Article  CAS  Google Scholar 

  15. Bisset, G. W., Clark, B. J., Haldar, J., Harris, M. C., Lewis, G. P., and Rocha e Silva, M., Brit. J. Pharmac. Chemother., 31, 537 (1967).

    Article  CAS  Google Scholar 

  16. Legros, J. J., Franchimont, P., and Hendrick, J. C., C. R. Seanc. Soc. Biol., 163, 2773 (1969).

    CAS  Google Scholar 

  17. Kornberg, A., in Methods in Enzymology, 1, 441 (Academic Press, New York, 1955).

    Book  Google Scholar 

  18. Matthews, E. K., in Calcium and Cellular Function (edit. by Cuthbert. A. W.), 163 (Macmillan, London, 1970).

    Book  Google Scholar 

  19. Katz, B., The Release of Neural Transmitter Substances (Liverpool University Press, 1969).

    Google Scholar 

  20. Thorn, N. A., Acta Endocrinol., 50, 357 (1965).

    Article  CAS  Google Scholar 

  21. Norstrom, A. Z., Zellforsch. Mikrosk. Anat., 129, 114 (1972).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Pharmacology, University of Cambridge, Cambridge

    E. K. MATTHEWS

  2. Radioimmunological Laboratory, Institute of Medicine, Hôpital de Bavière, Liège

    J. J. LEGROS

  3. Department of Physiology, University of Geneva Medical School, Geneva

    J. D. GRAU, J. J. NORDMANN & J. J. DREIFUSS

Authors
  1. E. K. MATTHEWS
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  2. J. J. LEGROS
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  3. J. D. GRAU
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  4. J. J. NORDMANN
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  5. J. J. DREIFUSS
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MATTHEWS, E., LEGROS, J., GRAU, J. et al. Release of Neurohypophysial Hormones by Exocytosis. Nature New Biology 241, 86–88 (1973). https://doi.org/10.1038/newbio241086a0

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  • DOI: https://doi.org/10.1038/newbio241086a0

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