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Interleukin 2 Immunoassay Using Monoclonal Antibodies

Bio/Technology volume 4, pages 983–986 (1986)Cite this article

Abstract

To design a sensitive and reproducible immunoassay for interleukin 2 (IL–2), two IL–2 reactive monoclonal antibodies (DMS–1 and DMS–5) were characterized extensively. The unique IL–2 binding characteristics of DMS 1 and DMS 5 were exploited to construct an IL–2 immunoassay that is sensitive to 10 pM murine and human IL–2 (150 pg/ml), amenable to both radioisotopic and enzymatic detection, and readily available to other laboratories.

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Author notes

  1. Ralph C. Budd

    Present address: Ludwig Institute for Cancer Research, Lausanne Branch, 1066, Epalinges, Switzerland

Authors and Affiliations

  1. The Department of Medicine, Dartmouth Medical School, Hanover, New Hampshire, 03756

    Ralph C. Budd & Kendall A. Smith

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  1. Ralph C. Budd
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  2. Kendall A. Smith
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Budd, R., Smith, K. Interleukin 2 Immunoassay Using Monoclonal Antibodies. Nat Biotechnol 4, 983–986 (1986). https://doi.org/10.1038/nbt1186-983

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  • DOI: https://doi.org/10.1038/nbt1186-983

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