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A cool way to make proteins

Nature Biotechnology volume 22, pages 826–827 (2004)Cite this article

Cold shock–inducible vectors enable the production of labeled proteins in bacteria for structural and functional studies.

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Figure 1: The pCold protein production system in E. coli.

Bob Crimi

References

  1. Remaut, E., Tsao, H. & Fiers, W. Gene 22, 103–113 (1983).

    Article  CAS  Google Scholar 

  2. Schein, C.H. & Noteborn, M.H.M. Bio/technology 6, 291–294 (1988).

    CAS  Google Scholar 

  3. Qing, G. et al. Nat. Biotechnol. 22, 877–882 (2004).

    Article  CAS  Google Scholar 

  4. Tanabe, H., Goldstein, J., Yang, M. & Inouye M. J. Bacteriol. 174, 3867–3873 (1992).

    Article  CAS  Google Scholar 

  5. Phadtare, S., Inouye, M. & Severinov, K. J. Mol. Biol. 337, 147–155 (2004).

    Article  CAS  Google Scholar 

  6. Mujacic, M., Cooper, K.W. & Baneyx, F. Gene 238, 325–332 (1999).

    Article  CAS  Google Scholar 

  7. Zartler, E.R. et al. J. Am. Chem. Soc. 125, 10941–10946 (2003).

    Article  CAS  Google Scholar 

  8. Klammt, C. et al. Eur. J. Biochem. 271, 568–580 (2004).

    Article  CAS  Google Scholar 

  9. Sawasaki, T., Ogasawara, T., Morishita, R. & Endo, Y. Proc. Natl. Acad. Sci. USA 99, 14652–14657 (2002).

    Article  CAS  Google Scholar 

  10. Galvao-Botton, L.M. et al. FEBS Lett. 552, 207–213 (2003).

    Article  CAS  Google Scholar 

  11. Schein, C.H. in The Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. (eds. Flickinger, M.C. & Drew, S.W.) 2156–2169 (John Wiley and Sons, London, 1999).

    Google Scholar 

Download references

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  1. Department of Human Biology, Sealy Center for Structural Biology, Chemistry and Genetics, 301 University Blvd., University of Texas Medical Branch in Galveston, Texas, 77555-0857, USA

    Catherine H Schein

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  1. Catherine H Schein
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Schein, C. A cool way to make proteins. Nat Biotechnol 22, 826–827 (2004). https://doi.org/10.1038/nbt0704-826

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