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Effects of MSC co-injection on the reconstitution of aplastic anemia patient following hematopoietic stem cell transplantation

Leukemia volume 24, pages 1791–1795 (2010)Cite this article

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Immunosuppressive therapy together with hematopoietic stem cell transplantation is used in the management of acquired severe aplastic anemia. However, despite significant improvement in these two treatment modalities, graft failure and graft-versus-host disease still remains the major challenge that needs to be addressed.1 Mesenchymal stem cells (MSCs) are adult multipotent cells capable of multipotential differentiation that possess immunosuppressive properties and are in use in the clinical setting to treat graft-versus-host disease.2, 3 Because of their fundamental function in maintaining HSC self-renewal and regulating hematopoiesis, MSC have also been used to facilitate donor HSC engraftment.4 In this study we report our experience with a severe aplastic anemia patient who failed to respond to three allogeneic hematopoietic stem cell transplantation, but after the co-administration of MSC fully recovered a hematopoietic system within 4 weeks and became transfusion independent. To investigate the role of MSC during the transplant and establish their fate after the hematopoietic recovery, we characterized the MSC isolated from the patient before (pre-transplant) and after (post transplant) his successful transplant and compared them with the normal MSC.

MSC were isolated from the patient's (recipient) BM samples collected pre- and post-fourth transplant and characterized morphologically and phenotypically. Unlike donor MSC, both pre- and post-transplant recipient MSC of the patient were smaller in size, morphologically heterogeneous, comprising round and flattened cells, as well as spindle-shaped cells. It is interesting that only lower level of CD105 and absence of CD90, CD106 and CD166 in both pre- and post-transplant MSC from the patient distinguished them from normal donor MSC.

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Acknowledgements

We thank Christopher Ridler for help with the animal experiments. These works were funded by Cancer Research UK and by European grant (contract No. LHSB-CT-2006-018817) to DB.

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  1. B G Jaganathan

    Present address: 5Current address: Department of Biotechnology, Indian Institute of Technology, Guwahati, India.,

Authors and Affiliations

  1. Haematopoietic Stem Cell laboratory, Cancer Research UK London Research Institute, London, UK

    B G Jaganathan & D Bonnet

  2. Department of Immunology—Hammersmith Hospital Imperial College, London, UK

    V Tisato & F Dazzi

  3. Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London, UK

    T Vulliamy & I Dokal

  4. Department of Haematology, King's College Hospital, London, UK

    J Marsh

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  1. B G Jaganathan
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Correspondence to D Bonnet.

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Jaganathan, B., Tisato, V., Vulliamy, T. et al. Effects of MSC co-injection on the reconstitution of aplastic anemia patient following hematopoietic stem cell transplantation. Leukemia 24, 1791–1795 (2010). https://doi.org/10.1038/leu.2010.164

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