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DelBank: a mouse ES-cell resource for generating deletions

Nature Genetics volume 28, pages 310–311 (2001)Cite this article

Chromosomal deletions are valuable reagents for identifying and mapping genetic function. To simplify the creation of deletions in mice, we developed a collection of embryonic stem (ES) cell clones called DelBank. DelBank is based upon a technology in which radiation-induced deletions of herpes simplex virus thymidine kinase (tk) cassettes, inserted by homologous recombination into F1 hybrid ES cells, are selected with the antiviral drug 1-2′-deoxy-2′-fluoro-β-D-arabinofuranosyl-5-iodouracil (FIAU)1,2. The deletions range in size from less than 1 cM to over 20 cM. Cells of the F1 generation retain germline competence following irradiation, and the heterozygosity enables the molecular mapping of deletion endpoints.

As DelBank clones are useful only if they retain germline colonizing capability, 10 parental lines were evaluated by us and DelBank users, and at least eight produced germline chimeras. To explore the extent of DelBank's utility, we successfully induced deletions and produced germline chimeras with a clone containing an insertion [Tg(tkneo)20Jcs] near the mouse counterpart of the Prader–Willi syndrome (PWS) critical region on chromosome 7.

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Figure 1: DelBank vector strategies.
Figure 2: DelBank insertions.

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Acknowledgements

This work was primarily supported by a grant from the Merck Genome Research Institute (to JCS). NCG was supported by a Training Grant (HD07065) from NIH.

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Authors and Affiliations

  1. The Jackson Laboratory, Bar Harbor, 04609, Maine, USA

    Neal C. Goodwin, Suzanne Hartford, Cate Wnek, Rebecca A. Bergstrom & John C. Schimenti

  2. Department of Experimental Pathology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan

    Yasumasa Ishida

  3. Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, Massachusetts, USA

    Philip Leder

Authors
  1. Neal C. Goodwin
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  2. Yasumasa Ishida
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  5. Rebecca A. Bergstrom
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  7. John C. Schimenti
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Correspondence to John C. Schimenti.

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Goodwin, N., Ishida, Y., Hartford, S. et al. DelBank: a mouse ES-cell resource for generating deletions. Nat Genet 28, 310–311 (2001). https://doi.org/10.1038/91064

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