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Targeting of non-lg sequences in place of the V segment by somatic hyper mutation

Nature volume 376, pages 225–229 (1995)Cite this article

Abstract

Affinity maturation of antibodies is characterized by localized hypermutation of the DNA around the V segment. Here we show, using mice containing single or multiple transgene constructs, that an immunoglobulin VK segment can be replaced by human β-globin or prokaryotic neo or gpt genes without affecting the rate of hypermutation; the V gene itself is not necessary for recruiting hypermutation. The ability to target hypermutation to heterologous genes in vivo could find more general applications in biology.

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Author notes

  1. F. Lozano

    Present address: Servicio de Immunologia, Hospital Clinico y Provi-cial de Barcelona, Vlillaroel 170, Barcelona, 08036, Spain

  2. Y. L. Chui

    Present address: Clinical Immunology Unit, Department of Medicine, Prince of Wales Hospital, Shatin, N.T., Hong Kong

Authors and Affiliations

  1. Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    J. Yélamos, N. Klix, B. Goyenechea, F. Lozano, Y. L. Chui, A. GonzÃlez FernÃndez, R. Pannell, M. S. Neuberger & C. Milstein

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  1. J. Yélamos
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  2. N. Klix
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  6. A. GonzÃlez FernÃndez
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  9. C. Milstein
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Yélamos, J., Klix, N., Goyenechea, B. et al. Targeting of non-lg sequences in place of the V segment by somatic hyper mutation. Nature 376, 225–229 (1995). https://doi.org/10.1038/376225a0

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