Abstract
WE describe a mutant human cell line (LBL 721.174)1,2 that has lost a function required for presentation of intracellular viral antigens with class I molecules of the major histocompatibility complex (MHC), but retains the capacity to present defined epitopes as extracellular peptides. The cell also has a defect in the assembly and expression of class I MHC molecules2,3,4, which we show can be restored by exposure of the cells to a peptide epitope. This phenotype suggests a defect in the association of intracellular antigen with class I molecules similar to that described for the murine mutant RMA-S (ref. 5), but in the present case the genetic defect can be mapped within the MHC locus on human chromosome 6.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
DeMars, R., Chang, C. C., Shaw, S., Reitnauer, P. J. & Sondel, P. M. Hum. Immun. 11, 77–97 (1984).
DeMars, R. et al. Proc. natn. Acad. Sci. U.S.A. 82, 8183–8183 (1985).
Shimizu, Y. et al. Mol. cell. Biol. 6, 1074–1087 (1986).
Salter, R. D. & Cresswell, P. EMBO J. 5, 943–949 (1986).
Townsend, A., Ohlen, C., Bastin, J., Foster, L. & Karre, K. Nature 340, 443–448 (1989).
Gotch, F., Rothbard, J. Howland, K., Townsend, A. & McMichael, A. Nature 326, 881–882 (1987).
Salter, R. D., Howell, D. N. & Cresswell, P. Immunogenetics 21, 235–235 (1985).
Whitehead, A. S., Colten, H. R., Chang, C. C. & DeMars, R. J. Immun. 134, 641–641 (1985).
Alexander, J., Payne, J. A., Murray, R., Frelinger, J. & Cresswell, P. Immunogenetics 29, 380–388 (1989).
Alexander, J., Payne, A. J., Shigekawa, B., Frelinger, J. & Cresswell, P. Immunogenetics 31, 169–178 (1990).
Ways, J. P., Rothbard, J. B. & Parham, P. J. Immun. 137, 217–222 (1986).
Townsend, A. R. M., Taylor, P. M., Mellor, A. L. & Askonas, B. A. Immunogenetics 17, 283–294 (1983).
Baez, M., Palese, P. & Kilbourne, E. D. J. infect. Dis. 141, 362–365 (1980).
Townsend, A. R. M. & Skehel, J. J. J. exp. Med. 160, 552–563 (1984).
Huet, S. et al. Int. Immun. (in the press).
Townsend A. R. M. et al. Cell 44, 959–968 (1986).
Owen, M. J., Kissonerghis, A. M. & Lodish, H. F. J. biol. Chem. 255, 9678–9684 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cerundolo, V., Alexander, J., Anderson, K. et al. Presentation of viral antigen controlled by a gene in the major histocompatibility complex. Nature 345, 449–452 (1990). https://doi.org/10.1038/345449a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/345449a0
This article is cited by
-
TCR-engineered T cells targeting E7 for patients with metastatic HPV-associated epithelial cancers
Nature Medicine (2021)
-
A personal retrospective on the mechanisms of antigen processing
Immunogenetics (2019)
-
Deficiency of activated STAT1 in head and neck cancer cells mediates TAP1-dependent escape from cytotoxic T lymphocytes
Cancer Immunology, Immunotherapy (2011)
-
Mouse strains with point mutations in TAP1 and TAP2
Immunology & Cell Biology (2010)
-
From influenza to HIV—and back?
Nature Immunology (2007)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.