Abstract
Morphological transformation of NIH/3T3 cells by transfection with DNA has been used to identify transforming sequences in human tumours1,2. Transforming activity has been reported for DNAs isolated from bladder, mammary, colon and lung carcinomas, neuroblastoma, lymphoid and myeloid tumours1–6. Each of these tissues seems to contain different transforming sequences except for the colon and lung tumours where the same sequence seems to be involved5. We now report that in two different human sarcoma cell lines, a fibrosarcoma and an embryonal rhabdomyosarcoma, the DNAs have transforming activity. The transforming gene is the same in both sarcomas but differs from the activated sequences detected in other tumours. We have also found that the transforming gene has no detectable homology to eight retrovirus oncogenes tested.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Shih, C., Padhy, L. C., Murray, M. & Weinberg, R. A. Nature 290, 261–264 (1981).
Krontiris, T. G. & Cooper, G. M. Proc. natn. Acad., Sci. U.S.A. 78, 1181–1184 (1981).
Lane, M.-A., Sainten, A. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 78, 5185–5189 (1981).
Murray, M. J. et al. Cell 25, 355–361 (1981).
Perucho, M. et al. Cell 27, 467–476 (1981).
Lane, M.-A., Sainten, A. & Cooper, G. M. Cell 28, 873–880 (1982).
Graham, F. L. & Van der Eb, A. J. Virol. 52, 456–467 (1973).
McAllister, R. M., Melnyk, J., Finklestein, J. Z., Adams, E. C. & Gardner, M. B. Cancer 24, 520–526 (1969).
Rasheed, S., Nelson-Rees, W. A., Toth, E. M., Arnstein, P. & Gardner, M. B. Cancer 33, 1027–1033 (1974).
Billiau, A. et al. Antimicrob. Ag. Chemother. 12, 11–15 (1977).
Goldfarb, M., Shimizu, K., Perucho, M. & Wigler, M. Nature 296, 404–409 (1982).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Gusella, J. F. et al. Proc. natn. Acad. Sci. U.S.A. 77, 2829–2833 (1980).
Rubin, C. M., Houck, C. M., Deininger, P. L. & Schmid, C. W. Nature 284, 372–374 (1980).
Parada, L. F., Tabin, C. J., Shih, C. & Weinberg, R. A. Nature 297, 474–478 (1982).
Der, C. J., Krontiris, T. G. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 79, 3637–3640 (1982).
Eva, A. et al. Nature 295, 116–119 (1982).
Pulciani, S. et al. Proc. natn. Acad. Sci. U.S.A. 79, 2845–2849 (1982).
Weiss, R. A., Teich, N., Varmus, H. E. & Coffin, J. RNA Tumor Viruses (Cold Spring Harbor Laboratory, New York, 1982).
Wahl, G. M., Stern, M. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 76, 3683–3687 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Marshall, C., Hall, A. & Weiss, R. A transforming gene present in human sarcoma cell lines. Nature 299, 171–173 (1982). https://doi.org/10.1038/299171a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/299171a0
This article is cited by
-
CD6-mediated inhibition of T cell activation via modulation of Ras
Cell Communication and Signaling (2022)
-
The path to the clinic: a comprehensive review on direct KRASG12C inhibitors
Journal of Experimental & Clinical Cancer Research (2022)
-
A perspective on the early days of RAS research
Cancer and Metastasis Reviews (2020)
-
Proteomic analysis of peritrophic membrane (PM) from the midgut of fifth-instar larvae, Bombyx mori
Molecular Biology Reports (2012)
-
The cytoplasmic protein GAP is implicated as the target for regulation by the ras gene product
Nature (1988)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.