Abstract
SISTER chromatid exchange (SCE) is a symmetrical exchange between a newly duplicated chromatid and its sister1. The demonstration that many agents that damage DNA also increase the frequency of SCE2 suggests that SCE is a reflection of a basic DNA repair process, and perhaps a cytological manifestation of post-replication repair of a recombinational character2–6. The question then arises as to the correlation between SCE and chromatid aberration, particularly chromatid interchange (CI), which also occurs as a consequence of exchange between newly duplicated chromatids7,8. To investigate whether these two types of exchange are due to the same molecular event, I have studied the reaction of SCE and CI to caffeine and cyclo-heximide in endoreduplication mitoses of cultured human embryonic skin fibroblasts. I report here evidence suggesting that the events involved are separate.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Taylor, J. H., Woods, P. S. & Hughes, W. L. Proc. natn. Acad. Sci. U.S.A. 43, 122–217 (1957).
Perry, P. & Evans, H. J. Nature 258, 121–125 (1975).
Kato, H. Expl Cell Res. 82, 383–390 (1973).
Kato, H. Expl Cell Res. 85, 239–247 (1974).
Wolff, S., Bodycote, J. & Painter, R. B. Mutat. Res. 25, 73–81 (1974).
Latt, S. A. Proc. natn. Acad. Sci. U.S.A. 71, 3162–2166 (1974).
Evans, H. J. in Recovery and Repair Mechanism in Radiobiology 111–133 (Brook-haven National Laboratory Associated Universities Inc., New York, 1967).
Kihlman, B. A. in Advances in Cell and Molecular Biology 59–107 (ed. DuPraw, E. J.)(Academic, New York and London, 1971).
Perry, P. & Wolff, S. Nature 251, 156–158 (1974).
Sasaki, M. S. Mutat. Res. 29, 433–448 (1975).
Cleaver, J. E. & Thomas, G. H. Biochem. biophys. Res. Commun. 36, 203–208 (1969).
Fujiwara, Y. Expl Cell Res. 75, 483–489 (1972).
Trosko, J. E. & Chu, E. H. Y. Chem. Biol. Interaction 6, 317–332 (1973).
Lehmann, A. R. & Kirk-Bell, S. Mutat. Res. 26, 73–82 (1974).
Nilsson, K. & Lehmann, A. R. Mutat. Res. 30, 255–266 (1975).
Lehmann, A. R. J. molec. Biol. 66, 319–337 (1972).
Hyodo, M., Koyama, H. & Ono, T. Expl Cell Res. 67, 461–463 (1971).
Fujiwara, Y. Cancer Res. 32, 2089–2095 (1972).
Gautzchi, J. R. & Kern, R. M. Expl Cell Res. 80, 15–26 (1973).
Pohjanpelto, P. Expl Cell Res. 102, 138–142 (1976).
Ceccarini, C. & Eagle, H. In Vitro 12, 346–351 (1976).
Wolff, S. & Bodycote, J. Mutat. Res. 29, 85–91 (1975).
Kato, H. Chromosoma 59, 179–191 (1977).
Chaganti, R. S. K., Schonberg, S. & German, J. Proc. natn. Acad. Sci. U.S.A. 71, 4508–4512 (1974).
Latt, S. A., Stetten, G., Juergens, L. A., Buchanan, G. R. & Gerald, P. S. Proc. natn. Acad. Sci. U.S.A. 72, 4066–4070 (1975).
Galloway, S. M. & Evans, H. J. Cytogenet. Cell Genet. 15, 17–29 (1975).
Wolff, S., Rodin, B. & Cleaver, J. E. Nature 265, 347–349 (1977).
Sasaki, M. S. Mutat. Res. 46, 152–153 (1977).
Kihlman, B. A. Chromosoma 51, 11–18 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SASAKI, M. Sister chromatid exchange and chromatid interchange as possible manifestation of different DNA repair processes. Nature 269, 623–625 (1977). https://doi.org/10.1038/269623a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/269623a0
This article is cited by
-
The diplochromosome of endoreduplicated cells: A new approach to highlight the mechanism of sister chromatid exchange
Chromosoma (1996)
-
Enhancement of genetic transformation frequencies of mammalian cell cultures by damage to the cell DNA
Molecular and General Genetics MGG (1985)
-
Induction of endoreduplication by hydrazine in Chinese hamster V 79 cells and reduced incidence of sister chromatid exchanges in endoreduplicated mitoses
Chromosoma (1984)
-
Relationship between spontaneous instability of chromosomes and sister chromatid exchanges in Fanconi's anemia
Bulletin of Experimental Biology and Medicine (1984)
-
Higher inductions of twin and single sister chromatid exchanges by cross-linking agents in Fanconi's anemia cells
Human Genetics (1982)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.