Abstract
THE preparation of plasmid DNA requires the collection of plasmid-containing bacteria, usually by centrifugation of liquid cultures. This step is a potential source of laboratory contamination, especially when large volumes, requiring continuous flow centrifugation, are processed.
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References
Adams, M. H., Bacteriophages (Interscience, New York, 1959).
Pauli, O., and Genth, H., Z. Lebensm. Unters. Forsch., 132, 216–227 (1966).
Hasenbank, R., et al., Molec. gen. Genet., 127, 1–18 (1973).
Herschfield, V., Boyer, H. W., Yanofsky, C., Lovett, M. A., and Helinski, D. R., Proc. natn. Acad. Sci. U.S.A., 71, 3455–3459 (1974).
Covey, C., Richardson, D., and Carbon, J., Molec. gen. Genet. (in the press).
Mandel, M., and Riga, A., J. molec. Biol., 53, 159–162 (1970).
Katz, L., Kingsbury, D. T., and Helinski, D. R., J. Bact., 114, 577–591 (1973).
Guerry, P., LeBlanc, D. J., and Falkow, S., J. Bact., 116, 1064–1066 (1973).
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WEISSMANN, C., BOLL, W. Reduction of possible hazards in the preparation of recombinant plasmid DNA. Nature 261, 428–429 (1976). https://doi.org/10.1038/261428a0
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DOI: https://doi.org/10.1038/261428a0
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