Abstract
THE development of a method for colony formation in vitro in semi-solid medium by normal macrophages and granulocytes1–3, has been a useful aid in elucidating the control of growth and differentiation of these two types of normal white blood cells and the changes in this control that occur in leukaemia and other diseases4–6. The formation of these two types of colonies requires the addition of an inducing protein, which we call MGI5, that is found in the appropriate serum or condition medium. This inducer does not induce the formation of lymphocyte colonies and it would obviously be of value to obtain colony formation in vitro by normal bone marrow-derived (B) and thymus-derived (T) lymphocytes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Pluznik, D., and Sachs, L., J. cell. comp. Physiol., 66, 319–324 (1965).
Ichikawa, Y., Pluznik, D. H., and Sachs, L., Proc. natn. Acad. Sci. U.S.A., 56, 488–495 (1966).
Bradley, T. R., and Metcalf, D., Aust. J. exp. Biol. med. Sci., 44, 287–300 (1966).
Fibach, E., Hayashi, M., and Sachs, L., Proc. natn. Acad. Sci. U.S.A., 70, 343–346 (1973).
Sachs, L., Harvey Lectures, 68, 1–35 (Academic, New York, 1974).
Lotem, J., and Sachs, L., Proc. natn. Acad. Sci. U.S.A., 71, 3507–3511 (1974).
Ginsburg, H., and Sachs, L., J. cell. comp. Physiol., 66, 199–220 (1965).
Mishell, R. I., and Dutton, R. W., J. exp. Med., 126, 423–442 (1967).
Greaves, M., and Janossy, G., Transplant. Rev., 11, 87–130 (1972).
Böyum, A., Scand. J. clin. Lab. Invest., 21, Suppl. 97 (1968).
Chen, C., and Hirsch, J. G., Science, 176, 60–61 (1972).
Broome, J. D., and Jeng, M. W., J. natn. Cancer Inst., 49, 579–581 (1972).
Stephenson, J. R., Axelrod, A. A., McLeod, D. L., and Shreeve, M. M., Proc. natn. Acad. Sci. U.S.A., 68, 1542–1546 (1971).
Rozenszajn, L. A., Shoham, D., and Kalechman, I., Immunology (in the press).
Lay, W. H., Mendes, N. F., Bianco, C., and Nussenzweig, V., Nature, 230, 531–532 (1971).
Wybran, J., Carr, M. C., and Fudenberg, H. H., J. clin. Invest., 51, 2537–2543 (1972).
Fröland, S. S., Scand. J. Immun., 1, 269–280 (1972).
Bianco, C., Patrick, R., and Nussenzweig, V., J. exp. Med., 132, 702–720 (1970).
Metcalf, D., et al., Nature, 255, 630–632 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
FIBACH, E., GERASSI, E. & SACHS, L. Induction of colony formation in vitro by human lymphocytes. Nature 259, 127–129 (1976). https://doi.org/10.1038/259127a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/259127a0
This article is cited by
-
Stem cells and diversity
Leukemia (2003)
-
Functional characterization of T lymphocytes grown in semisolid agar
Blut (1983)
-
Impaired T-lymphocyte colony formation by cord blood mononuclear cells
Journal of Clinical Immunology (1982)
-
Suppression of human T-cell colony formation during pregnancy
Nature (1978)
-
Control of normal cell differentiation and the phenotypic reversion of malignancy in myeloid leukaemia
Nature (1978)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
