Abstract
THE hypophysiotropic hypothalamus regulates the secretion of the anterior pituitary gland through the production and release of specific peptide hormones. Luteinising hormone-releasing hormone (LHRH) and thyrotrophin-releasing hormone (TRH) are two such peptides which have been fully characterised and synthesised1,2. Studies on intact animals have revealed the importance of various neurotransmitters and hormones in the modulation of hypothalamic LHRH and TRH secretion but little is known of the cellular mechanisms involved in such control, especially at the neurosecretory nerve terminal. We have described3,4 the use of nerve endings (synaptosomes) isolated from the mammalian hypothalamus to investigate factors which influence the release of corticotrophin-releasing factor (CRF). As this factor is uncharacterised, a major limitation of this work has been the use of bioassay techniques to measure indirectly corticotrophin-releasing activity. In the study described here radioimmunoassays have been used to measure the release of immunoreactive LHRH and TRH from synaptosomes prepared from rat and sheep hypothalami, and we report the influence of neurotransmitters and steroid hormones on these processes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Matsuo, H., Baba, Y., Nair, R. M. G., Arimura, A., and Schally, A. V., Biochem. biophys. Res. Commun., 43, 1344 (1971).
Folkers, K., Enzmann, F., Bøler, J., Bowers, C. Y., and Schally, A. V., Biochem. biophys. Res. Commun., 37, 123 (1969).
Edwardson, J. A., and Bennett, G. W., Nature, 251, 425 (1974).
Bennett, G. W., and Edwardson, J. A., J. Endocr., 65, 33 (1975).
Jeffcoate, S. L., Fraser, H. M., Holland, D. T., and Gunn, A., Acta Endocr., 75, 625 (1974).
Jeffcoate, S. L., Fraser, H. M., Gunn, A., and White, N., J. Endocr., 59, 191 (1973).
Schneider, H. P. G., and McCann, S. M., Endocrinology, 87, 249 (1970).
Schneider, H. P. G., and McCann, S. M., J. Endocr., 46, 555 (1970).
Schneider, H. P. G., and McCann, S. M., Endocrinology, 85, 121 (1969).
Kamberi, I. A., Schneider, H. P. G., and McCann, S. M., Endocrinology, 86, 278 (1969).
Grimm, Y., and Reichlin, S., Endocrinology, 93, 626 (1973).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BENNETT, G., EDWARDSON, J., HOLLAND, D. et al. Release of imnumoreactive luteinising hormone-releasing hormone and thyrotrophin-releasing hormone from hypothalamic synaptosomes. Nature 257, 323–325 (1975). https://doi.org/10.1038/257323a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/257323a0
This article is cited by
-
Neuroendocrine regulation of thyrotropin-releasing hormone (TRH) in the tuberoinfundibular system
Journal of Endocrinological Investigation (1993)
-
The effects of histamine on the concentrations of immunoreactive thyrotropin-releasing hormone in the stomach and hypothalamus in rats
Gastroenterologia Japonica (1990)
-
Interrelationship of monoamines and luliberin in the synaptosomes of the medial-basal hypothalamus and its individual structures
Neuroscience and Behavioral Physiology (1986)
-
New concepts in the regulation of hypothalamic gonadotropin releasing hormone (GnRH) secretion
Journal of Endocrinological Investigation (1986)
-
Dopamine decreases release of luteinizing hormone releasing hormone from superfused rat mediobasal hypothalamus
Journal of Endocrinological Investigation (1985)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.