Abstract
As in higher eukaryotes1,2, the nuclear DNA of the simple eukaryote Saccharomyces cerevisiae is replicated from multiple internal initiation sites along the chromosome3–5. The very small size of the chromosomal DNA in yeast3,6,7 and the availability of temperature-sensitive DNA synthesis mutants8,9 make a detailed study of DNA replication easier in yeast than in most other eukaryotic systems. In this report, nuclear DNA molecules from several DNA synthesis mutants which had been classified as DNA initiation or DNA propagation mutants on the basis of incorporation kinetics were examined with the electron microscope. Our results provide independent criteria for the classification of DNA initiation and propagation mutants on the basis of the structure of the nuclear DNA at the restrictive temperature.
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PETES, T., NEWLON, C. Structure of DNA in DNA replication mutants of yeast. Nature 251, 637–639 (1974). https://doi.org/10.1038/251637a0
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DOI: https://doi.org/10.1038/251637a0
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