Abstract
IT is known that in biological systems histamine is broken down by histaminase1. In this reaction oxygen is consumed and hydrogen peroxide is formed. A mano-metric method of measurement2 is based on the oxygen consumption and a chemical method3,4 on the production of hydrogen peroxide. In the latter method indigo is added to the incubation mixture and the discoloration of this mixture by the hydrogen peroxide formed is a measure of the enzyme activity. This is due to the peroxidatic activity of catalase mostly present in the biological fluids or tissue extracts under test. The disadvantages of the chemical method are the long incubation period of 24 h and the necessity of at least 1 ml. of serum in the case of histaminase determinations in blood.
Similar content being viewed by others
Article PDF
References
Zeller, E. A., in The Enzymes, 2, (i), 536 (Academic Press, New York, 1951).
Zeller, E. A., and Birkhäuser, H., Schweiz, med. Wschr., 70, 975 (1940).
Zeller, E. A., Schweiz. med. Wschr., 71, 1349 (1941).
Kapeller-Adler, R., Biochem. J., 48, 99 (1951).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
AARSEN, P., KEMP, A. Rapid Spectrophotometric Micromethod for Determination of Histaminase Activity. Nature 204, 1195 (1964). https://doi.org/10.1038/2041195a0
Published:
Issue Date:
DOI: https://doi.org/10.1038/2041195a0
This article is cited by
-
Neurohumoral alterations and their role in amoebiasis
Indian Journal of Clinical Biochemistry (2005)
-
An improved spectrophotometric assay for histamine and diamine oxidase (DAO) activity
Agents and Actions (1985)
-
Investigation of the copper content in the active site of ceruloplasmin
Pharmaceutical Chemistry Journal (1983)
-
Determination of histaminase (diamine oxidase) activity byo-dianisidine test: Interference of ceruloplasmin
Agents and Actions (1977)
-
Histamine and its metabolism in mammals part II: Catabolism of histamine and histamine liberation
Agents and Actions (1975)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.