Abstract
IN a recent report1 the spectra of various dipeptides in the isoionic, basic, and acidic states in water were examined by transmission techniques with ‘Irtran-2’ cells, and the use of such spectra for structural and identification purposes was discussed. A drawback of the method used is that no information can be obtained in areas where water absorbs very strongly. This is particularly true around 6µ (∼ 1,660 cm−1), where amino-acids and peptides should show characteristic bands. The principle of attenuated total reflectance2 has enabled the examination of even comparatively weak absorption bands and has permitted spectra to be obtained on difficult samples. Spectra of sucrose in water and of whole blood have been shown by manufacturers of infra-red equipment.
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References
Parker, F. S., Fed. Proc., 22, 478 (1963). Spectral data were presented in Atlantic City, New Jersey, April 18, 1963, at the meeting of the Fed. Amer. Soc. Exp. Biol.
Fahrenfort, J., Spectrochim. Acta, 17, 698 (1961). The author states, “The similarity between the new ATR spectra and transmission spectra is so great in all cases investigated that probably the collections of standard transmission spectra can also be used as a reference for identification by means of the reflection spectra”.
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PARKER, F. Attenuated Total Reflectance Spectra of Glycyl-L-alanine in Aqueous Solutions. Nature 200, 1093–1094 (1963). https://doi.org/10.1038/2001093a0
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DOI: https://doi.org/10.1038/2001093a0
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