Abstract
THERE are many occasions in biochemical work in which inorganic phosphorus must be determined in the presence of some acid-labile phosphorus compounds such as glucose-l-phosphate or adenosine triphosphate. In Allen's method1, for example, molybdenum blue colour is developed in 0.5 N perchloric acid. Owing to high acidity of the medium, glucose-1-phosphate or adenosine triphosphate is more or less hydrolysed during colour development and interferes with the determination.
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Allen, R. J. L., Biochem. J., 34, 858 (1940).
Whelan, W. J., and Bailey, J. M., Biochem. J., 58, 560 (1954).
Weil-Malherbe, W., and Green, R. H., Biochem. J., 49, 286 (1951).
Weil-Malherbe, W., Biochem. J., 55, 741 (1953).
Nakamura, M., Nippon Nôgei-Kagaku Kaishi (J. Agric. Chem. Soc. Japan), 24, 1 (1950).
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NAKAMURA, M., MORI, K. Colorimetric Determination of Inorganic Phosphorus in the Presence of Glucose-I-phosphate and Adenosine Triphosphate. Nature 182, 1441 (1958). https://doi.org/10.1038/1821441a0
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DOI: https://doi.org/10.1038/1821441a0
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