Abstract
IN the procedure for the quantitative analysis of sugars, described by Flood, Hirst and Jones1, the paper strips containing the sugars are cut out and extracted by allowing condensed water to run down the paper. It has been found, however, that destruction of certain sugars occurs on boiling a solution under these conditions. To obviate this difficulty, we have devised a technique of extraction at room temperature similar to that used by Dent2 for amino-acids.
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References
Flood, A. E., Hirst, E. L., and Jones, J. K. N., J. Chem. Soc., 1679 (1948).
Dent, C. E., Biochem. J., 41, 240 (1947).
Hirst, E. L., Hough, L., Jones, J. K. N., J. Chem. Soc., 928 (1949).
Hirst, E. L., Jones, J. K. N., J. Chem. Soc., 1659 (1949).
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LAIDLAW, R., REID, S. Filter-Paper Chromatography: Extraction of Sugars from the Paper at Room Temperature. Nature 166, 476–477 (1950). https://doi.org/10.1038/166476b0
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DOI: https://doi.org/10.1038/166476b0
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