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Molecular cloning and characterization of a novel human protein phosphatase 2C cDNA (PP2C ε)

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Abstract

We have isolated a novel cDNA from the human fetal brain cDNA library with homology to the Mg2+-dependent serine/threonine protein phosphatase 2C (PP2C) family. The cDNA is 3055 bp in length, and the predicted coding region encodes a 360-amino-acid protein, which shows 99% identity to the PP2C ε from rat and mouse. Then we term it human PP2C ε gene. The gene is mapped to chromosome 3q26.1 and contains 4 exons. RT-PCR analysis shows that the PP2C ε is widely expressed in human tissues and the expression levels in heart, placenta, lung, liver, kidney, and pancreas are relatively high.

Abbreviations: PP2Cε– Protein Phospatase 2C ε; RT-PCR – reverse transcriptionalPCR; SAPKs – stress-activated protein kinases; MAPK – mitogen--activated protein kinase.

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of life Sciences, Fudan University, Shanghai, 200433, People's Republic of China

    Feng Jin, Chaoneng Ji, Lingfeng Liu, Jianfeng Dai, Shaohua Gu, Xianfei Sun, Yi Xie & Yumin Mao

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  1. Feng Jin
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  2. Chaoneng Ji
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  3. Lingfeng Liu
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  4. Jianfeng Dai
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  5. Shaohua Gu
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  6. Xianfei Sun
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  7. Yi Xie
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  8. Yumin Mao
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Jin, F., Ji, C., Liu, L. et al. Molecular cloning and characterization of a novel human protein phosphatase 2C cDNA (PP2C ε). Mol Biol Rep 31, 197–202 (2004). https://doi.org/10.1023/B:MOLE.0000043624.96006.eb

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  • DOI: https://doi.org/10.1023/B:MOLE.0000043624.96006.eb

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