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Microfluidic ELISA: On-Chip Fluorescence Imaging

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Abstract

Fluorescent reactions of a heterogeneous sandwich enzyme-linked immunoassay (ELISA) in an all-PDMS [poly (dimethylsiloxane)] microfluidic device were detected using a cooled charge coupled device (CCD) camera interfaced with an epifluorescence microscope. The study represents preliminary efforts to integrate biochemical reactions and detection on-chip using the “hybrid” detection approach. In initial experiments, the PDMS chip microsensor was successfully used to quantify a model analyte (sheep IgM) with sensitivity down to 17 nM. Thus, we demonstrate here the extension of this hybrid integrated technique to on-chip imaging and quantification of light emission from a biochemical immunoassay in PDMS chip.

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Correspondence to Edward Eteshola.

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Eteshola, E., Balberg, M. Microfluidic ELISA: On-Chip Fluorescence Imaging. Biomedical Microdevices 6, 7–9 (2004). https://doi.org/10.1023/B:BMMD.0000013360.65653.c2

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  • DOI: https://doi.org/10.1023/B:BMMD.0000013360.65653.c2

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