biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:141-144, 2004 | DOI: 10.1023/B:BIOP.0000024292.51938.aa

Iron Chlorosis in Grafted Sweet Orange (Citrus sinensis L.) Plants: Physiological and Biochemical Responses

V. Chouliaras1, I. Therios1,*, A. Molassiotis1, G. Diamantidis2
1 Department of Agriculture, Laboratory of Pomology, Aristotle University of Thessaloniki, Thessaloniki, Greece
2 Department of Agriculture, Laboratory of Agricultural Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece

Fe deficiency was imposed in Citrus sinensis L. cultivars Valencia and New Hall grafted on C. aurantium and Swingle citrumelo rootstocks by the absence of Fe (-Fe) or by the presence of bicarbonate in the Hoagland nutrient solution. In Fe-deprived leaves total and active Fe concentration, and peroxidase and catalase activities were decreased while the ratios carotenoids/chlorophylls, P/Fe, and K/Ca were increased. Fe(III) chelate reductase activity was induced in (-Fe)-treated roots whereas it was depressed in bicarbonate-treated roots.

Keywords: carotenoids; catalase; chlorophylls; Fe(III) chelate reductase; peroxidase
Subjects: carotenoids, iron deficiency; catalase; chelate reductase; chlorophyll, iron deficiency; Citrus aurantium; Citrus sinensis; Fe(III) chelate reductase; grafting; iron chlorosis, photosynthetic pigments, enzymes; nutrient medium, Hoagland and Arnon; peroxidase; rootstock, sweet orange; sweet orange, rootstocks; Swingle citrumelo

Published: March 1, 2004  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Chouliaras, V., Therios, I., Molassiotis, A., & Diamantidis, G. (2004). Iron Chlorosis in Grafted Sweet Orange (Citrus sinensis L.) Plants: Physiological and Biochemical Responses. Biologia plantarum48(1), 141-144. doi: 10.1023/B:BIOP.0000024292.51938.aa
Download citation

References

  1. Albano, J.P., Miller, W.B.: Iron deficiency stress influences physiology of iron acquisition in marigold (Tagetes erecta L.).-J. amer. Soc. hort. Sci. 121: 438-441, 1996. Go to original source...
  2. Alcantara, E., Romero, F.J., De la Guardia, M.M.: Characterization of the tolerance to iron chlorosis in different peach rootstocks grown in nutrient solution. I. Effect of bicarbonate and phosphate.-Plant Soil 130: 115-119, 1988. Go to original source...
  3. Almansa, M.S., Hernandez, J.A., Jimenez, A., Botella, M.A., Sevilla, F.: Effects of salt stress on superoxide dismutase activity in leaves of Citrus limonum in different rootstockscion combinations.-Biol. Plant. 45: 545-549, 2002. Go to original source...
  4. Britton, G.: Carotenoids.-In: Charlwood, B.V., Banthorpe, D.V. (ed.): Methods in Plant Biochemistry. Pp. 493-515. Academic Press, London 1991.
  5. Fernandez-Lopez, J.A., Almela, L., Lopez-Roca, J.M., Alcaraz, C.: Iron deficiency in Citrus limon: effects on protochlorophyllase synthetic pigments and chlorophyllase activity.-J. Plant Nutr. 14: 1133-1144, 1991. Go to original source...
  6. Fernandez-Lopez, J.A., Lopez-Roca, J.M., Almela, L.: Mineral composition of iron chlorotic Citrus limon L. leaves.-J. Plant Nutr. 16: 1395-1407, 1993. Go to original source...
  7. Guzman, M., Urrestazaru, M., Romero, L.: Active and total iron in Castanea sativa and their relation to other nutrients.-J. Plant Nutr. 9: 909-921, 1986. Go to original source...
  8. Hoagland, D.R., Arnon, D.L.: The water culture method for growing plants without soil.-Calif. agr. Exp. Sta. Circular 347: 1-32, 1950.
  9. Mengel, K., Breininger, T., Bulb, W.: Bicarbonate, the most important factor inducing iron chlorosis in vine grapes on calcareous soils.-Plant Soil 81: 333-344, 1984. Go to original source...
  10. Moog, P.R., Bruggemann, W.: Iron reductase systems on the plasma membrane-Plant Soil 165: 241-260, 1994. Go to original source...
  11. Morales, F., Grasa, R., Abadia, A., Abadia, J.: Iron chlorosis paradox in fruit trees.-J. Plant Nutr. 21: 815-825, 1998. Go to original source...
  12. Nenova, V.R, Stoyanov, I.G.: Effects of some growth regulators on young iron deficient maize plants.-Biol. Plant. 43: 35-39, 2000. Go to original source...
  13. Ngo, T., Lenhoff, H.: A sensitive and versatile chromogenic assay for peroxidase and peroxidase-coupled reactions.-Anal. Biochem. 105: 389-397, 1980. Go to original source...
  14. Ranieri, A., Castagna, A., Baldan, B., Soldatini, G.F.: Iron deficiency differently affects peroxidase isoforms in sunflower.-J. exp. Bot. 52: 25-35, 2001. Go to original source...
  15. Romera, F.J., Alcantara, E., De La Guardia, M.D.: Characterization of the tolerance to iron chlorosis in different peach rootstocks grown in nutrient solution. II. Iron-stress mechanisms.-Plant Soil 130: 121-125, 1991. Go to original source...
  16. Wang, C.Y.: Effect of temperature preconditioning on catalase, peroxidase and superoxide dismutase in chilled zucchini squash.-Postharvest Biol. Technol. 5: 67-76, 1995. Go to original source...
  17. Welch, R.M., Norvell, W.A., Schaefer, S.C., Shaff, J.E., Kochian, L.V.: Induction of iron(III) and copper(II) reduction in pea (Pisum satinum L.) roots by Fe and Cu status: Does the root-cell plasmalemma Fe(III)-chelate reductase perform a general role in regulating cation uptake?-Planta 190: 555-561, 1993. Go to original source...
  18. Wintermans, J.F., Mots, A.: Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol.-Biochim. biophys. Acta 109: 448-453, 1965. Go to original source...