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Differential Effects of Cell Density on 5-Lipoxygenase (5-LO), Five-Lipoxygenase-Activating Protein (FLAP) and Interleukin-1β (IL-1β)-Expression in Human Neutrophils

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Abstract

We have analyzed the effect of cellular density of 5-Lipoxygenase (5-LO), 5-lipoxygenase-activating protein (FLAP) and interleukin-1β (IL-1β) gene expression in neutrophils from healthy subjects under culture conditions of low and high cell density. By using RT-PCR techniques, we have found that 5-LO mRNA accumulation decreased in cells cultured at high density, while FLAP mRNA is not affected. De novo 5-LO synthesis, as well as steady-state levels, were reduced in cells maintained at high density. In contrast, the high density conditions lead to the induction of IL-1β gene at the RNA and protein levels as measured by RT-PCR and by immunoprecipitation. These results suggest that cellular density plays a role in gene modulation when neutrophils are accumulating at an inflammatory site since neutrophils obtained from the synovial fluid of patients with RA exhibit a protein synthesis profile similar to that observed in peripheral blood neutrophils cultured at high density.

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  1. Josée Gauthier

    Present address: Virology Department, Glaxo Wellcome, 5 Moore Drive, Research Triangle Park, North Carolina, 27704

Authors and Affiliations

  1. Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599

    Christian Jobin

  2. Division of Rheumatology, University of North Carolina, Chapel Hill, North Carolina, 27599

    Josée Gauthier

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Jobin, C., Gauthier, J. Differential Effects of Cell Density on 5-Lipoxygenase (5-LO), Five-Lipoxygenase-Activating Protein (FLAP) and Interleukin-1β (IL-1β)-Expression in Human Neutrophils. Inflammation 21, 235–250 (1997). https://doi.org/10.1023/A:1027326405788

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