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One Stomach-One Strain (Does Helicobacter pylori Strain Variation Influence Disease Outcome?)

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Abstract

The aim of the study was to determine inter- andintrapatient variation of Helicobacter pylori strainsbased on genomic fingerprinting and cagA(cytotoxin-associated gene A) status. Ten bacterialcolonies from each of 10 patients with gastric cancer(GC), 10 with duodenal ulcer (DU), and 10 with gastritis(GI) were used. The presence of the putative adhesingene, the cagA gene, and the strain specific banding pattern obtained by arbitrary primed (AP-) PCRwas analyzed. Genomic fingerprinting showed extensiveinterpatient variation, but the banding patternsobtained from colonies from the same patient were always identical (intrapatient variation). In fivepatients, the cagA status varied between the coloniesdespite identical banding patterns. Among patients in adeveloped country such as Sweden, the proportion with multiple-strain infection of H. pylori islow, but subclones with differing cagA status existwithin the strain.

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Authors
  1. Helena Enroth
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  2. Olof Nyren
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  3. Lars Engstrand
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Enroth, H., Nyren, O. & Engstrand, L. One Stomach-One Strain (Does Helicobacter pylori Strain Variation Influence Disease Outcome?). Dig Dis Sci 44, 102–107 (1999). https://doi.org/10.1023/A:1026658301825

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