Abstract
Growth-arrest and DNA-damage inducible (GADD) genes and Myeloid differentiation primary response (MyD) genes represent a family of genes that play a key role in negative control of cell growth. In the present study, following clone and location of human GADD45 γ (MyDL) gene, we have found that its mRNA expression level was down-regulated in 15/23 cases of clinic hepatocellular carcinoma (HCC) by comparing the northern hybridization results between the tumor tissues and adjacent normal tissues. Transient transfection of GADD45 γ cDNA with intact open reading frame sequence into the human hepatoma cells Hep-G2 resulted in dramatic growth suppression in colony formation assays. Furthermore, flow cytometry analysis indicated that GADD45 γ caused cell cycle arrest at G2/M transition when transfected into Hep-G2 cells. Therefore, the possible role of GADD45 γ in cell growth control was further confirmed in this paper.
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References
Liebermann DA & Hoffman B (1998) Oncogene 17: 3319–3329
Liebermann DA & Hoffman B (2002) Leukemia 16: 527–541
Fornace Jr AJ, Alamo IJ & Hollander MC (1988) Proc. Natl. Acad. Sci. USA 85: 8800–8804
Zhan Q, Lord KA, Alamo IJ, Hollander MC, Carrier & Ron D (1994) Mol. Cell. Biol. 14: 2361–2371
Fornace Jr AJ (1992) Annu. Rev. Genet. 26: 507–526
Takekawa M & Saito H (1998) Cell 95: 521–530
Zhang W, Bae I, Krishnaraju K, Azam N, Fan W, Smith K, Hoffman B & Liebermann DA (1999) Oncogene 18: 4899–4907
Beadling C, Johnson KW & Smith KA (1993) Proc. Nat. Acad. Sci. 90: 2719–2723
Takekawa M & Saito H (1998) Cell 95: 521–530
Suzuki M, Watanabe TK, Fujiwara T, Nakamura Y, Takahashi E & Tanigami A (1999) J. Hum. Genet. 44: 300–303
Gong R, Yu L, Zhang H, Tu Q, Zhao Y, Yang J, Xu Y & Zhao S (2000) Cytogenet. Cell Genet. 88: 95–96
Zhan Q, Antinore MJ, Wang XW, Carrier F, Smith ML & Harris CC (1999) Oncogene 18: 2892–2900
King RW, Jackson PK & Kirschner MW (1994) Cell 79: 563–571
Wang XW, Zhan Q, Coursen JD, Khan MA, Kontny HU & Yu L (1999) Proc. Natl. Acad. Sci. USA 96: 3706–3711
Pisani P, Parkin DM & Ferlay J (1993) Int J Cancer 55: 891–903
Tabor E & Kobayashi K (1992) J Natl Cancer Inst 84: 86–90
Brechof C, Gozuacik D, Murakami Y & Brechof PP (2000) Cancer Biol 10: 211–231
Moradpour D & Blum HE (2000) Zentralbl Chir 125: 592–596
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Sun, L., Gong, R., Wan, B. et al. GADD45γ, down-regulated in 65% Hepatocellular Carcinoma (HCC) from 23 Chinese patients, inhibits cell growth and induces cell cycle G2/M arrest for Hepatoma Hep-G2 cell lines. Mol Biol Rep 30, 249–253 (2003). https://doi.org/10.1023/A:1026370726763
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DOI: https://doi.org/10.1023/A:1026370726763