Abstract
The feasibility of electrotransformation of Frankia strain EuI1c was investigated. Cell viability decreased as the capacitance of the electrical pulse increased. The addition of 10% glycerol to the electroporation buffer increased cell viability rates. At a fixed capacitance, cell viability was voltage-dependent and resistance-dependent. Electroporation with a high capacitance was mutagenic and produced antibiotic-resistant cells. Electroporation of Frankia strain EAN1pec chromosomal DNA into strain EuI1c generated several colonies that were resistant to lincomycin or kasugamycin.
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Myers, A.K., Tisa, L.S. Effect of electroporation conditions on cell viability of Frankia EuI1c. Plant and Soil 254, 83–88 (2003). https://doi.org/10.1023/A:1024986611913
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DOI: https://doi.org/10.1023/A:1024986611913