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Qualitative and quantitative composition of pigments in Phaeodactylum tricornutum (Bacillariophyceae) stressed by iron

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Abstract

The effect of Fe(III) deficiency on qualitative and quantitative changes in pigment composition in Phaeodactylum tricornutum Bohlin was demonstrated by HPLC and AAS. Maximum content of pigments showed the diatom cells incubated at the optimum iron concentration, i.e., 10 μM. The contents of chlorophyll a, chlorophyll c 1+c 2, fucoxanthin, diadinoxanthin and β,β-carotene were 109.99, 20.16, 40.39, 1.29 and 1.48 fg per cell, respectively. The results obtained showed that Fe(III) affected qualitative and quantitative pigment composition in P. tricornutum. The content of individual pigments, proportions between accompanying pigments and their ratios to chlorophyll a were important indicators of phytoplankton response to iron stress. The strong reduction in β,β-carotene content, several times (2–5) increase in diadinoxanthin level as compared to β,β-carotene, and high amount of diadinoxanthin in relation to chlorophyll a were observed in algae growing at very low Fe(III) concentrations, 0.001 and 0.01 μM. The data suggested that phytoplankton pigments could be a potential physiological marker.

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Authors and Affiliations

  1. Institute of Oceanology, Polish Academy of Sciences, Powstańców Warszawy 55, 81-712, Sopot, Poland

    Alicja Kosakowska, Jolanta Lewandowska & Joanna Stoń

  2. Institute of Oceanology, Polish Academy of Sciences, Powstańców Warszawy 55, 81-712, Sopot, Poland

    Krystyna Burkiewicz

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  1. Alicja Kosakowska
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  2. Jolanta Lewandowska
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  3. Joanna Stoń
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  4. Krystyna Burkiewicz
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Kosakowska, A., Lewandowska, J., Stoń, J. et al. Qualitative and quantitative composition of pigments in Phaeodactylum tricornutum (Bacillariophyceae) stressed by iron. Biometals 17, 45–52 (2004). https://doi.org/10.1023/A:1024452802005

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