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Oral administration of milk fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 to DBA/1 mice inhibits secretion of proinflammatory cytokines

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Abstract

We have previously shown that oral administration of skimmed milk(SM) fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 (OLL1073R-1/SM) to DBA/1 mice inhibited the development of collagen-induced arthritis (CIA). In this study, our aim was to examine possible mechanisms of inhibiting the development of CIA. We studied the effect of OLL1073R-1/SM on cytokine secretion from cells of popliteal lymph nodes (lymph node cells; LNC) of mice. The results showed that feeding OLL1073R-1/SM inhibited secretion of proinflammatory cytokines such as interferon γ (IFN-γ), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and the chemokine, monocyte chemoattractant protein 1 (MCP-1). The most prominent effect was inhibition of TNF-α. Secretion of IL-2 and IL-4 were not influenced. Feeding OLL1073R-1/SM inhibited secretion of proinflammatory cytokines produced by accessory cells, but not T cells. We conclude that CIA may be prevented via down regulation of secretion of proinflammatory cytokines such as IL-6, TNF-α and IFN-γ, and of the chemokine of MCP-1.

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References

  • Alonzi T, Fattori E, Lazzaro D, Costa P, Probert L, Kollias G, Benedetti FD, Poli V and Ciliberto G (1998) Interleukin 6 is required for the development of collagen-induced arthritis. J Exp Med 187: 461–468.

    Article  PubMed  CAS  Google Scholar 

  • Boissier MC, Chiocchia G, Bessis N, Hajnal J, Garotta G, Nicoletti F and Fournier C (1995) Biphasic effect of interferon-γ in murine collagen-induced arthritis. Eur J Immunol 25: 1184–1196.

    PubMed  CAS  Google Scholar 

  • Cooper SM, Sriram S and Ranges GE (1988) Suppression of murine collagen-induced arthritis with monoclonal anti-Ia antibodies and augmentation with IFN-γ. J Immunol 141: 1958–1962.

    PubMed  CAS  Google Scholar 

  • Feldmann M, Brennan FM and Maini RN (1996) Role of cytokines in rheumatoid arthritis. Annu Rev Immunol 14: 397–440.

    Article  PubMed  CAS  Google Scholar 

  • Haller D, Blum S, Bode C, Hammes WP and Schiffrin EJ (2000) Activation of human peripheral blood mononuclear cells by non-pathogenic bacteria in vitro: Evidence of NK cells as primary targets. Infect Immun 68: 752–759.

    Article  PubMed  CAS  Google Scholar 

  • Joosten LAB, Helsen MMA, Saxne T, Van de Loo FAJ, Heinegård D and Van den Berg WB (1999) IL-1αβ blockade prevents cartilage and bone destruction in murine type II collagen-induced arthritis, whereas TNF-α blockade only ameliorates joint inflammation. J Immunol 163: 5049–5055.

    PubMed  CAS  Google Scholar 

  • Joosten LAB, Helsen MMA, Van de Loo FAJ and Van den Berg WB (1996) Anticytokine treatment of established type II collageninduced arthritis in DBA/1 mice. A comparative study using anti-TNF-α, anti-IL-1-α/β, and IL-1Ra. Arthritis Rheum 39: 797–809.

    PubMed  CAS  Google Scholar 

  • Kano H, Kaneko T and Kaminogawa S (2002) Oral intake of Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1 prevents collagen-induced arthritis in mice. J Food Prot 65: 153–160.

    PubMed  Google Scholar 

  • Kato I, Endo-Tanaka K and Yokokura T (1998) Suppressive effects of the oral administration of Lactobacillus casei on type II collagen-induced arthritis in DBA/1 mice. Life Sciences 63: 635–644.

    Article  PubMed  CAS  Google Scholar 

  • Kitazawa H, Itoh T, Tomioka Y, Mizugaki M and Yamaguchi T (1996) Induction of IFN-β and IL-1α production in macrophages stimulated with phosphopolysaccharide produced by Lactococcus lactis ssp. cremoris. Int J Food Microbiol 31: 99–106.

    Article  PubMed  CAS  Google Scholar 

  • Marin ML, Tejada-Simon MV, Murtha J, Ustunol Z and Pestka JJ (1997) Effects of Lactobacillus spp. on cytokine production by RAW264.7 macrophage and EL-4 thymoma cell lines. J Food Prot 60: 1364–1370.

    CAS  Google Scholar 

  • Matsuzaki T, Yamazaki R, Hashimoto S and Yokokura T (1998) The effect of oral feeding of Lactobacillus casei strain Shirota on immunoglobulin E production in mice. J Dairy Sci 81: 48–53.

    Article  PubMed  CAS  Google Scholar 

  • Mauritz NJ, Holmdahl R, Jonsson R, Van der Meide PH, Scheynius A and Klareskog L (1988) Treatment with gamma-interferon triggers the onset of collagen arthritis in mice. Arthritis Rheum 31: 1297–1304.

    PubMed  CAS  Google Scholar 

  • Miettinen M, Matikainen S, Vuopio-Varkila J, Pirhonen J, Varkila K, Kurimoto M and Julkunen I (1998) Lactobacilli and Streptococci induce interleukin-12 (IL-12), IL-18, and gamma interferon production in human peripheral blood mononuclear cells. Infect Immun 66: 6058–6062.

    PubMed  CAS  Google Scholar 

  • Mosmann TR and Sad S (1996) The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol Today 17, 138–146.

    Article  PubMed  CAS  Google Scholar 

  • Onozaki K, Matsushima K, Aggarwal B and Oppenheim JJ (1985) Human interleukin-1 is a cytocidal factor for several tumor cell lines. J Immunol 135: 3962–3968.

    PubMed  CAS  Google Scholar 

  • Orme IM and Cooper AM (1999) Cytokine/chemokine cascades in immunity to tuberculosis. Immunol Today 20: 307–312.

    Article  PubMed  CAS  Google Scholar 

  • Parks E, Strieter R, ukacs NW, Gauldie J, Hitt M, Graham FL and Kunkel SL (1998) Transient gene transfer of IL-12 regulates chemokine expression and disease severity in experimental arthritis. J Immunol 160: 4615–4619.

    PubMed  CAS  Google Scholar 

  • Ramsay AJ, Ruby J and Ramshaw IA (1993) A case for cytokines as effector molecules in the resolution of virus infection. Immunol Today 14: 155–157.

    Article  PubMed  CAS  Google Scholar 

  • Rangavajhyala N, Shahani KM, Sridevi G and Srikumaran S (1997) Nonlipopolysaccharide component(s) of Lactobacillus acidophilus stimulate(s) the production of interleukin-1α and tumor necrosis factor-α by murine macrophages. Nutr Cancer 28: 130–134.

    Article  PubMed  CAS  Google Scholar 

  • Sato K, Saito H and Tomioka H (1988) Enhancement of host resistance against Listeria infection by Lactobacillus casei: Activation of liver macrophages and peritoneal macrophages by Lactobacillus casei. Microbiol Immunol 32: 689–698.

    PubMed  CAS  Google Scholar 

  • Solis-Pereyra B, Aattouri N and Lemonnier D (1997) Role of food in the stimulation of cytokine production. Am J Clin Nutr 66: 521S–525S.

    PubMed  CAS  Google Scholar 

  • Solis-Pereyra B and Lemonnier D (1993) Induction of human cytokines by bacteria used in dairy foods. Nutr Res 13: 1127–1140.

    Article  Google Scholar 

  • Thornton S, Boivin GP, Kim KN, Finkelman FD and Hirsch R (2000) Heterogeneous effects of IL-2 on collagen-induced arthritis. J Immunol 165: 1557–1563.

    PubMed  CAS  Google Scholar 

  • Trinchieri G, Wysocka M, D'Andrea A, Rengaraju M, Aste-Amezaga M, Kubin M, Valiante NM and Chehimi J (1992) Natural killer cell stimulatory factor (NKSF) or interleukin-12 is a key regulator of immune response and inflammation. Prog Growth Factor Res 4: 355–368.

    Article  PubMed  CAS  Google Scholar 

  • Urban JL, Shephard HM, Rothstein JL, Sugarman BJ and Schreiber H (1986) Tumor necrosis factor: A potent effector molecule for tumor cell killing by activated macrophages. Proc Natl Acad Sci USA 83: 5233–5237.

    Article  PubMed  CAS  Google Scholar 

  • Williams RO, Feldmann M and Maini RN (1992) Anti-tumor necrosis factor ameliorates joint disease in murine collagen-induced arthritis. Proc Natl Acad Sci USA 89: 9784–9788.

    Article  PubMed  CAS  Google Scholar 

  • Wooley PH, Dutcher J, Widmer MB and Gillis S (1993) Influence of a recombinant human soluble tumor necrosis factor receptor FC fusion protein on type II collagen-induced arthritis in mice. J Immunol 151: 6602–6607.

    PubMed  CAS  Google Scholar 

  • Wooley PH, Luthra HS, Stuart JM and David CS (1981) Type II collagen-induced arthritis in mice. I. Major histocompatibility complex (I region) linkage and antibody correlates. J Exp Med 154: 688–700.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Food Functionality Research Institute, Meiji Dairies Co., 540 Naruda, Odawara, Kanagawa, Japan

    Hiroshi Kano, Orie Mogami & Masayuki Uchida

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  1. Hiroshi Kano
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  2. Orie Mogami
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  3. Masayuki Uchida
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Correspondence to Hiroshi Kano.

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Kano, H., Mogami, O. & Uchida, M. Oral administration of milk fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 to DBA/1 mice inhibits secretion of proinflammatory cytokines. Cytotechnology 40, 67–73 (2002). https://doi.org/10.1023/A:1023966121989

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