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Indirect cytotoxicity evaluation of pseudowollastonite

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Abstract

This study aimed to evaluate the cytotoxicity of substances leached by pseudowollastonite (CaSiO3). It has been previously shown that calcium (Ca2+) and silicate (SiO3 −) ions are released from pseudowollastonite into biological solutions. Both of these ions are known to influence the biological metabolism of osteoblastic cells essential in the mineralization process and bone-bonding mechanism. The indirect toxicity evaluation was performed by extraction method, according to International Standard Organization (ISO). Pseudowollastonite pellets obtained by solid-state reaction were incubated, in culture medium, during 24, 48, 72 or 168 h at different concentrations (5, 10, 15, 50, 100, 200 mg/ml). The cytotoxicity of each extract in presence of human osteoblastic cell line (SaOS-2) was quantitatively assessed by measuring the viability (succinate dehydrogenase activity, MTT), the membrane integrity (the uptake of the neutral red by viable cells, NR) as well as the cell necrosis by measuring the lactate dehydrogenase (LDH) released in the culture medium. No significant alteration of membrane integrity or cell suffering was detectable. However, increased cell metabolism was observed for cells exposed to pseudowollastonite extract with longest extraction time (168 h). In conclusion, mineral elements leached by pseudowollastonite do not significantly affect the metabolism of osteoblastic cells.

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Authors and Affiliations

  1. Orthopaedic Research Laboratory, Université Catholique de Louvain, Avenue Mounier, 53, B 1200, Brussels, Belgium

    D. Dufrane & C. Delloye

  2. Department of Adult Oral Health, St Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, University of London, Mile End Road, London, E1 4NS, UK

    I. J. McKay

  3. Departamento de Ciencia y Tecnología de Materiales, Universidad Miguel Hernandez, Edificio Torrevaillo Avda, Ferrocarril s/n, 03202, Elche (Alicante), Spain

    P. N. De Aza

  4. Instituto de Cerámica y Vidrio ICV-CSIC, Crta de Valencia Km, 24.3., 28500, Arganda del Rey, Madrid, Spain

    S. De Aza

  5. Cell Biochemical Laboratory, Université Catholique de Louvain, B 1348, Louvain-La-Neuve, Belgium

    Y. J. Schneider

  6. Laboratoire de RMN, Université de, Mons-Hainaut, Mons, Belgium

    M. Anseau

Authors
  1. D. Dufrane
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  2. C. Delloye
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  4. P. N. De Aza
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  5. S. De Aza
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  7. M. Anseau
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Dufrane, D., Delloye, C., McKay, I.J. et al. Indirect cytotoxicity evaluation of pseudowollastonite. Journal of Materials Science: Materials in Medicine 14, 33–38 (2003). https://doi.org/10.1023/A:1021545302732

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