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Spermine Inhibits [3H]Glycine Binding at the NMDA Receptors from Plexiform Layers of Chick Retina

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Abstract

Saturable specific binding of glycine to synaptosomal membranes from plexiform layers of the retina has been described, which seems to correspond to the modulatory site on NMDA-receptors (26). Spermine inhibited specific [3H]glycine binding to membranes from synaptosomal fractions from the outer (P1) and the inner (P2) plexiform layers of 1–3 day-old chick retinas in a dose-dependent manner with an IC50 = 35 μM for the P1 fraction and 32 μM for the P2 fraction. Kinetic experiments and non-linear regression analysis of [3H]glycine-specific binding showed a Kd ~ 100–150 nM in both fractions, and a higher Bmax (4.11 ± 0.47 pmol/mg protein) for the inner plexiform layer compared to the outer plexiform layer (Bmax = 2.76 ± 0.25 pmol/mg protein). Strychnine-insensitive [3H]glycine binding was inhibited by 100 μM spermine, due to a reduction in Bmax (P1 = 0.84 ± 0.16 pmol/mg protein; P2 = 0.81 ± 0.16 pmol/mg protein) without affecting the Kd. Association and dissociation constants in the absence and presence of 50 μM spermine remained unchanged. Results demonstrate the presence of a single modulatory site for spermine on NMDA receptors, in both synaptic layers of the chick retina.

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  1. Departamento de Neurociencias, Universidad Nacional Autonoma de México (UNAM), Instituto de Fisiología Celular, Mexico

    Froylan Calderón & Ana María López-Colomé

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  1. Froylan Calderón
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  2. Ana María López-Colomé
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Calderón, F., López-Colomé, A.M. Spermine Inhibits [3H]Glycine Binding at the NMDA Receptors from Plexiform Layers of Chick Retina. Neurochem Res 23, 1363–1369 (1998). https://doi.org/10.1023/A:1020794421783

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