Abstract
Progress has been made in establishing a genetic transformation model for Laminaria japonica (Phaeophyta, Laminariales). The model includes introduction of foreign genes by biolistic bombardment, use of promoter SV40 to drive gene expression, algal regeneration by parthenogenesis and selection by chloramphenicol or hygromycin.
Similar content being viewed by others
References
Fang, Z., J. Dai, J. Cui & Y. Ou, 1978. The first record of female sporophytes of Laminaria japonica. Chin. Sci. Bull. 23: 43–44.
Goddijn, O. J. M. & J. Pen, 1995. Plants as bioreactors. Trends Biotechnol. 13: 379–387.
Haq, T. A., 1995. Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science 268: 714–716.
Henry, E. C. & R. H. Meints, 1994. Recombinant viruses as transformation vectors of marine macroalgae. J. appl. Phycol. 6: 247–253.
Kuebler, J. E., S.C. Minocha & A. C. Mathieson, 1994. Transient expression of the GUS reporter gene in protoplasts of Porphyra miniata(Rhodophyta). J. mar. Biotechnol. 1: 165–169.
Lewis, R. J., B. Y. Jiang, M. Neushul & X. G. Fei, 1993. Haploid parthenogenetic sporophytes of Laminaria japonica(Phaeophyceae). J. Phycol. 29: 363–369.
Li, X., 1998. Studies on genetic transformation of Laminaria japonica(Phaeophyta) by using female gametophytes as gene recipients. Master Thesis, Institute of Oceanology, Chinese Academy of Sciences, 49 pp.
Moffat, A. S., 1995. Exploring transgenic plants as a new vaccine source. Science 268: 658–660.
Notoya, M., M. Nagashima & Y. Aruga, 1992. Influence of light intensity and temperature on callus development in young sporophytes of four species of Laminariales (Phaeophyta). Kor. J. Phycol. 7: 101–107.
Notoya, M., M. Nagashima & Y. Aruga, 1994. Influence of light intensity and temperature on callus development in young sporophytes of three species of Laminariales (Phaeophyta). J. mar. Biotechnol. 2: 15–18.
Qin, S., P. Jiang, X. Li & C. K. Tseng, 1998a. The expression of lacZin regenerated sporophytes of parthenogenetic Laminaria japonica. Proceedings of the 2nd Asia-Pacific Marine Biotechnology Conference and the 3rd Asia-Pacific Conference on Algal Biotechnology: 205–208.
Qin, S., J. Wu, X. Wang, X. Li, P. Jiang & C. K. Tseng, 1998b. The expression of foreign genes in Laminaria japonica(Phaeophyta). In Morton, B. (ed.), The Marine Biology of South China Sea. Hong Kong University Press, Hong Kong: 209–217.
Qin, S., J. Zhang, W. Li, X. Wang, S. Tong, Y. Sun & C. K. Tseng, 1994. Transient expression of GUS gene in phaeophytes using biolistic particle delivery system. Oceanol. Limnol. Sin. 25: 353–356 (in Chinese with English abstract).
Qin, S., X. Wang, S. Tong and C. K. Tseng, 1995. Isolation and culture of female haploid protoplasts from Laminaria japonica. Oceanol. Limnol. Sin. 26 (suppl.): 126–129 (in Chinese with English abstract).
Saga, N., 1991. Protoplasts and somatic hybridization-controversal discussion ‘No’ side. Proceedings of a COST-48 Workshop, Spain: 25–30.
Saga, N. & Y. Sakai, 1984. Isolation of protoplasts from Laminariaand Porphyra. Bull. jap. Soc. Sci. Fish. 50:1085.
Tseng, C. K. & S. Qin, 1991. Marine algal biotechnology in China: present conditions and prospects. Proceeding of International Symposium on Biotechnology of Salt Ponds, Tianjin: 61–68.
Wang, X., S. Qin, X. Li, P. Jiang, C. K. Tseng & M. Qin, 1998a. Effects of ultrasonic treatment on female gametophytes of Laminaria japonica(Phaeophyta). Chin. J. Oceanol. Limnol. 16 (Suppl.): 62–66.
Wang, X., S. Qin, X. Li, P. Jiang, C. K. Tseng & M. Qin, 1998b. High efficient induction of callus and regeneration of sporophytes in Laminaria japonica(Phaeophyta). Chin. J. Oceanol. Limnol. 16 (Suppl.): 67–74.
Wu, J., 1996. Study of selectable markers and expression of foreign genes in Laminaria japonicaMaster Thesis, Institute of Oceanology, Chinese Academy of Sciences, 39 pp. (in Chinese with English abstract).
Zhang, L., L. Cheng, J. Yuan, C. Li, S. Jia, N. Xu & N. Zhao, 1991. Ultrasonic direct gene transfer – the establishment of high efficiency genetic transformation system for tobacco. Sci. Agric. Sin. 24: 83–89 (in Chinese with English abstract).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Qin, S., Sun, GQ., Jiang, P. et al. Review of genetic engineering of Laminaria japonica (Laminariales, Phaeophyta) in China. Hydrobiologia 398, 469–472 (1999). https://doi.org/10.1023/A:1017091629539
Issue Date:
DOI: https://doi.org/10.1023/A:1017091629539