Abstract
Moss and lichen samples from the region of the Bulgarian base on Livingston Island, Antarctica were examined for the presence of yeasts. Six pure cultures were obtained. They were screened for β-glucosidase production and two of them were selected. These were identified as Cryptococcus albidus AL2 and C. albidus AL3, according to their morphology, reproductive behaviour, and growth at different temperatures, salt concentrations, nutritional characteristics and various biochemical tests. These strains were examined for biosynthesis of β-glucosidase on different carbon sources under aerobic conditions. High exocellular and endocellular activities were obtained when they were grown on cellobiose, methyl-β-D-glucopyranoside and salicin. The time course of growth and β-glucosidase production of the yeast was examined by cultivation in a medium with cellobiose under aerobic conditions at temperatures 18 and 24 °C for 96 h. Cryptococcus albidus AL2 and C. albidus AL3 synthesized exocellular enzyme, respectively 58.33 and 55.83 U/ml and endocellular enzyme 137.75 and 205.34 U/ml at 24 °C for 72 h of the cultivation.
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References
Barnett, J.A., Payne, R.W. & Yarrow D. 1990 Yeasts: Characteristics and Identification, 2nd edn. Cambridge: Cambridge University Press. ISBN 0-521 35056-5.
Barron, N., Marchant, R., McHale L. & McHale, A.P. 1995 Partial characterization of β-glucosidase activity produced by Kluyveromyces marxianus IMB3 during growth on cellobiose containing media at 45 °C. Biotechnology Letters 10, 1047–1050.
Freer, S.N. 1991 Fermentation and aerobic metabolism of cellodextrins by yeast. Applied and Environmental Microbiology 3, 655–659.
Goto, S., Sugiyama, J. & Iizuka, H. 1969 A taxonomic study of antarctic yeasts. Mycologia 61, 748–774.
Gueguen, Y., Chemardin, P., Arnaud, A. & Galzy, P. 1994 Purification and characterization of the endocellular β-glucosidase of a new strain of Candida entomophila isolated from fermenting agave (Agave sp.) juice. Biotechnology and Applied Biochemistry 20, 185–198.
Gueguen, Y., Chemardin, P., Arnaud, A. & Galzy, P. 1995 Comparative study of extracellular and intracellular β-glucosidase of a new strain Zygosaccharomyces bailii isolated from fermenting agave juice. Journal of Applied Bacteriology 78, 270–280.
Herman, A. & Halvorson, H.O. 1962 Genetic control of β-glucosidase synthesis on Saccharomyces lactis. Journal of Bacteriology 85, 901–910.
Janbon, G., Arnaud, A. & Galzy, P. 1994 Selection and study of a Candida molischiana mutant derepressed for β-glucosidase production. FEMS Microbiology Letters 118, 207–212.
Kilian, S., Bernard, P., Potgieter, H & Preez, J. 1983 Utilization of glucose and cellobiose by Candida wickerhamii. Applied Microbiology and Biotechnology 17, 281–286.
Kreger-van Rij, N.J.W. 1984 The Yeasts: a Taxonomic Study. Elsevier science Publishing, Amsterdam. ISBN 0-444-80421-8.
Kurtzman, C.P. & Fell, J.W. 1998 The Yeasts: a Taxonomic Study, 4th edn. Elsevier Scientific Publisher, Amsterdam (Netherlands). ISBN 0-444-81312-8.
Leclerc, M., Gonde, P., Arnaud, A., Ratomahemina, R., Galzy, P. & Nicolas, M. 1984 The enzyme system in a strain of Candida wickerhamii Meyer and Yarrow participating in the hydrolysis of cellodextrins. Journal of General and Applied Microbiology 30, 509–521.
Margesin, R. & Schinner, F. 1999a Cold-Adapted Organisms. Springer, Heidelberg, Germany. ISBN 3-540-64973-5.
Margesin, R. & Schinner, F. 1999b Biotechnological Applications of Cold-Adapted Organisms. Springer, Heidelberg, Germany. ISBN 3-540-64972-7.
Mateo, J.J. & Stefano, R.D. 1997 Description of the β-glucosidase activity of a wine yeasts. Food Microbiology 14, 583–591.
McQuillan, A.M. & Halvorsen, H.O. 1963 Physiological changes occurring in yeast undergoing glucose repression. Journal of Bacteriology 84, 31–36.
Pavlova, K., Grigorova, D., Hristozova, Ts. & Angelov, A. 2001 Yeast strains from the Livingston Island, Antarctica. Folia Microbiologica 46, 4.
Ray, M., Shivaji, S., Rao Shyamala, N. & Bhargova, P. 1989 Yeast strains from the Schirmarcher oasis Antarctica. Polar Biology 9, 305–309.
Riccio, P., Rossano, R., Vinella, M., Domizio, P., Zito, F., Sansevrino, F., D'Elia, A. & Rosi, V. 1999 Extraction and immobilization in one step of two β-glucosidase released from a yeast strain of Debaryomyces hansenii. Enzyme and Microbial Technology 24, 123–129.
Rosi, I., Vinella, M. & Domizio, P. 1994 Characterization of β-glucosidase activity in yeast of oenological origin. Journal of Applied Bacteriology 77, 519–527.
Saha, B.C. & Bothast, R.J. 1996 Glucose tollerant and thermophilic β-lucosidase from yeast. Biotechnology Letters 18, 155–158.
Savova, I. 1999 Isolation and taxonomic investigation of yeast strains from the Antarctic. Bulgarian Antarctic Research, Life Sciences 2, 13–18.
Vasserot, Y., Chemardin, P., Arnaud, A. & Galzy, P. 1990 Evidence for the β-glucosidase activity and cellobiose fermentation by various Kloeckera strains. Acta Biotechnologica 5, 451–457.
Vasserot, Y., Chemardin, P., Arnaud, A. & Galzy, P. 1991 Purification and properties of a new strain of Candida molischiana able to work at low pH values: possible use in the liberation of bound terpenols. Journal of Basic Microbiology 31, 301–312.
Vishniac, H.S. & Kurtzman, C.P. 1992 Cryptococcus antarcticus sp. Nov. and Cryptococcus albidosimilis sp. Nov., basidioblastomycetes from Antarctic soils. International Journal of Systematic Bacteriology 4, 547–553.
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Pavlova, K., Angelova, G., Savova, I. et al. Studies of Antarctic yeast for β-glucosidase production. World Journal of Microbiology and Biotechnology 18, 569–573 (2002). https://doi.org/10.1023/A:1016337529488
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DOI: https://doi.org/10.1023/A:1016337529488