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Expression of G1 Phase Cyclins in Human Gastric Cancer and Gastric Mucosa of First-Degree Relatives

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Abstract

The aim of this study was to investigate the expression of D-type cyclins and cyclin E in gastric cancer patients (N = 34), in healthy first-degree relatives of gastric cancer patients (N = 29), and in control subjects (N = 18). Expression of cyclins D1, D2, D3, and E was determined by RT-PCR. Localization of cyclin expression was determined by immunohistochemistry. Expression of cyclins D2, D3, and E was more frequently detected in tumor tissue compared with tumor-free gastric mucosa (P < 0.05) and was associated with the presence of intestinal metaplasia. In contrast, cyclin D1 was frequently expressed in both tumor- and tumor-free tissue. Cyclin D3 expression was more frequently detected in the antrum mucosa of first-degree relatives compared to controls (P < 0.01) and was associated with the presence of Helicobacter pylori. Our data suggest that deregulation of G1 phase cyclins may play a role in gastric carcinogenesis, and may point to the presence of molecular alterations in individuals at an increased risk for gastric cancer.

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Authors and Affiliations

  1. Medical Department I, Gastroenterology, Hematology & Oncology, Technical University Hospital, Dresden, Germany

    Stephan Miehlke, Jun Yu, Daniel Szokodi, Gerhard Ehninger & Ekkehard Bayerdörffer

  2. Department of Gastroenterology, Hepatology, and Infectious Diseases, Otto-von-Guericke University, Magdeburg, Germany

    Matthias Ebert & Peter Malfertheiner

  3. Klinikum Bayreuth, Institute of Pathology, D-95445, Bayreuth, Germany

    Michael Vieth & Manfred Stolte

  4. Department of Surgery, Technical University Hospital, Dresden, Germany

    Eberhard Kuhlisch & Ursula Wehrmann

  5. Institute for Medical Statistics and Biometry, Technical University, Dresden, Germany

    Rene Buchcik

  6. Department of Gastroenterology, City Hospital, Dresden-Neustadt, Germany

    Wolfgang Schimmin

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  1. Stephan Miehlke
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Miehlke, S., Yu, J., Ebert, M. et al. Expression of G1 Phase Cyclins in Human Gastric Cancer and Gastric Mucosa of First-Degree Relatives. Dig Dis Sci 47, 1248–1256 (2002). https://doi.org/10.1023/A:1015358127751

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