Skip to main content
SpringerLink
Log in

Optimization of glycerol feeding for clavulanic acid production by Streptomyces clavuligerus with glycerol feeding

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Glycerol at 10–20 g l−1 increased clavulanic acid production by Streptomyces clavuligerus in shake-flask culture. The biosynthesis of clavulanic acid continued for longer by feeding glycerol and production increased to 250 mg l−1 compared with 115 mg l−1 without feeding. In fermenter batch culture, degradation of clavulanic acid began after 72 h. With glycerol feeding in fed-batch culture, clavulanic acid production was not only increased further to about 280 mg l−1 but also remained stable up to 130 h.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aharonowitz Y, Demain AL (1978) Catabolite regulation of cephalosporin production in Streptomyces clavuligerus. Antimicrob. Agents Chemother. 14: 159-164.

    Google Scholar 

  • Brown AG, Butterworth D, Cole M, Hanscomb G, Hood JD, Reading C, Rolinson GN (1976) Naturally-occurring α-lactamase inhibitors with antibacterial activity. J. Antibiot. 29: 668-669.

    Google Scholar 

  • Elson SW, Oliver RS (1978) Studies on the biosynthesis of clavulanic acid. I. Incorporation of 13C-labelled precursors. J. Antibiot. 31: 586-592.

    Google Scholar 

  • Foulstone M, Reading C (1982) Assay of amoxicillin and clavulanic acid, the components of Augmentin, in biological fluids with high-performance liquid chromatography. Antimicrob. Agents Chemother. 22: 753-762.

    Google Scholar 

  • Kirk S, Avignone-Rossa CA, Bushell ME (2000) Growth limiting substrate affects antibiotic production and associated metabolic flues in Streptomyces clavuligerus. Biotechnol. Lett. 22: 1803-1809.

    Google Scholar 

  • Liras P, Rodriguez-Garcia A (2000) Clavulanic acid, a α-lactamase inhibitor: biosynthesis and molecular genetics. Appl. Microbiol. Biotechnol. 54: 467-475.

    Google Scholar 

  • Mayer AF, Deckwer WD (1996) Simultaneous production and decomposition of clavulanic acid during Streptomyces clavuligerus cultivations. Appl. Microbiol. Biotechnol. 45: 41-46.

    Google Scholar 

  • Romero J, Liras P, Martin JF (1984) Dissociation of cephamycin and clavulanic acid biosynthesis in Streptomyces clavuligerus. Appl. Microbiol. Biotechnol. 20: 318-325.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan

    Kuo-Cheng Chen, Yun-Huin Lin, Cheng-Min Tsai & Chen-Hao Hsieh

  2. Department of Chemical Engineering, I-Shou University, Kaohsiung County, Taiwan

    Jer-Yiing Houng

Authors
  1. Kuo-Cheng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yun-Huin Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cheng-Min Tsai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chen-Hao Hsieh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jer-Yiing Houng
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, KC., Lin, YH., Tsai, CM. et al. Optimization of glycerol feeding for clavulanic acid production by Streptomyces clavuligerus with glycerol feeding. Biotechnology Letters 24, 455–458 (2002). https://doi.org/10.1023/A:1014553109425

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1014553109425

Search

Navigation