Abstract
The objectives of this study were to test whether freeze-dried microalgae are nutritionally adequate for rearing rotifers as food for gilthead seabream larvae. The elemental composition (C, N, H) and fatty acid composition were analysed in larvae of gilthead seabream, Sparus aurata L., rotifers Brachionus plicatilis and Brachionus rotundiformis and freeze-dried microalgae Nannochloropsis oculata. Four larval feeding treatments were tested: (A) larvae fed rotifers cultivated with freeze-dried microalgae and daily addition of freeze-dried microalgae to the larval tanks; (B) larvae fed rotifers cultivated with freeze-dried microalgae and daily addition of live microalgae to the larval tanks; (C) larvae fed rotifers cultivated with freeze-dried microalgae, without addition of microalgae to the larval tank and (D) larvae fed rotifers cultivated with live microalgae and daily addition of live microalgae to the larval tanks. No significant differences were observed between the biochemical composition of larvae with treatment A (with freeze-dried microalgae) and the composition of larvae in treatment D that were obtained with the acceptable methods for culture systems (with live microalgae).
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References
Ben-Amotz, A., R. Fisher & A. Schneller, 1987. Chemical composition of dietary species of marine unicellular algae and rotifer with emphasis on fatty acids. Mar. Biol. 95: 31–36.
Flynn, K. J., 1993. Carbon–nitrogen relations during batch growth of Nannochloropsis oculata (Eustigmatophyceae) under alternating light and dark. J. appl. Phycol. 5: 465–475.
Frolov, A. V., S. L. Pankov, K. N. Geradze, S. A. Pankova & L. V. Spektorova, 1991. Influence of the biochemical composition of food on the biochemical composition of the rotifer Brachionus plicatilis. Aquaculture 97: 181–202.
Guillard, R. R. L.& J. H. Ryther, 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervaceae (Cleve) Gran. Can. J. Microbiol. 8: 229–239.
Hardy, R. W., 1989. Diet Preparation. In Fish Nutrition. Academic Press, Inc., San Diego: 476–544.
Harris, R. K., T. Nishiyama & A. J. Paul, 1986. Carbon, nitrogen and caloric content of eggs, larvae and juveniles of the walleye pollock, Theragra chalcogramma. J. Fish Biol. 29: 87–98.
Izquierdo, M. S., T. Watanabe, T. Takeuchi, T. Arakawa & C. Kitajima, 1989. Requeriments of larval red sea bream Pagrus major for essential fatty acids. Nippon Suisan Gakkaishi 55: 859–867.
Kiørboe, T., P. Munk & K. Richardson, 1987. Respiration and growth of larval herring Clupea harengus: relation between specific dynamic action and growth efficiency. Mar. Ecol. Prog. Ser. 40: 1–10.
Kjørsvik, E., Y. Olsen, G. Roselund & O. Vadstein, 1991. Effect of various lipid enrichments in rotifers on the development of early stages in turbot. Larvi' 91 – Fish and Crustacean Larviculture Symposium. Europ. Aquacult. Soc. Sp. Pub. 15: 73–74.
Koven, W. M., G. W. Kissil & A. Tandler, 1989. Lipid and n-3 requirement of Sparus aurata larvae during starvation and feeding. Aquaculture 79: 185–191.
Koven, W. M., A. Tandler, G. W. Kissil, D. Sklan, O. Friezlander & M. Harel, 1990. The effect of dietary (n-3) polyunsaturated fatty acids on growth, survival and swim bladder development in Sparus aurata larvae. Aquaculture 91: 131–141.
Lubzens, E., A. Marko & A. Tietz, 1985. De novo synthesis of fattyacids in the rotifer Brachionus plicatilis. Aquaculture 47: 27–37.
May, R. C., 1971. Effects of delayed feeding on larvae of the grunion, Leuresthes tenuis. Fish. Bull. U.S. 69(2): 411–425.
Mourente, G., L. M. Lubian & J. M. Odriozola, 1990. Total fatty acid composition as a taxonomic index of some marine microalgae used as food in marine aquaculture. Hydrobiologia 203: 147–154.
Mourente, G., A. Rodriguez, D. R. Tocher & J. R. Sargent, 1993. Effects of dietary docohexaenoic acid (DHA; 22:6 n-3) on lipid and fatty acid composition and growth in gilthead sea bream (Sparus aurata L.) larvae during first feeding. Aquaculture 112: 79–98.
Navarro, N., 1996. Cultivo del rotífero Brachionus plicatilis O. F. Müller alimentado con microalgas liofilizadas. Dinámica de la población y calidad nutritiva como alimento larvario de peces marinos. PhD Thesis, University of Cádiz (Spain).
Navarro, N. & M. YÚfera, 1999. Influence of the food ration and individual density on production efficiency of semicontinuous cultures of Brachionus fed microalgae dry powder. Hydrobiologia 387/388: 483–487.
Navarro, N. & C. Sarasquete, 1998. Use of freeze-dried microalgae for rearing gilthead seabream, Sparus aurata, larvae. I. Growth, histology and water quality. Aquaculture 167: 179–193.
Reitan, K. I., J. R. Rainuzzo, G. Oie, & Y. Olsen, 1993. Nutritional effects of algal addition in first-feeding of turbot (Scophthalmus maximus L.) larvae. Aquaculture 118, 257–275.
Rodríguez, C., J. A. Pérez, M. S. Izquierdo, J. Mora, A. Lorenzo & H. Fernández-Palacios, 1993. Essential fatty acid requeriments for larval gilthead sea bream (S. aurata). Aqua. Fish. Manag. 24:295–304.
Salhi, M., M. S. Izquierdo, C. M. Hernández-Cruz, M. Gonzalez & H. Fernández-Palacios, 1994. Effect of lipid and n-3 HUFA levels in microdiets on growth, survival and fatty acid composition of larval gilthead seabream (Sparus aurata). Aquaculture 124: 275–282.
Salonen, K., J. Sarvala, I. Hakala & M.-L. Viljanen, 1976. The relation of energy and organic carbon in aquatic invertebrates. Limnol. Oceanogr. 21(5): 724–730.
Tucker, J. W., 1989. Energy utilization in bay anchovy, Anchoa mitchilli, and black sea bass, Centropristis striata striata, eggs and larvae. Fish. Bull. U.S. 87: 279–293.
Viso, A.-C. & J.-C. Marty, 1993. Fatty acids from microalgae. Phytochemistry 34(6): 1521–1533.
Watanabe, T., 1982. Lipid nutrition in fish. Comp. Biochem. Physiol. 1: 3–15.
Watanabe, T., C. Kitajima & S. Fujita, 1983. Nutritional values of live organisms used in Japan for mass propagation of fish: a review. Aquaculture 34: 115–143.
YÚfera, M., E. Pascual & J. Guinea, 1993a. Some factors influencing the biomass of the rotifer Brachionus plicatilis in culture. Hydrobiologia 255/256: 159–164.
YÚfera, M., A. Polo & E. Pascual, 1993b. Changes in chemical composition and biomass during the transition from endogenous to exogenous feeding of Sparus aurata L. (Pisces, Sparidae) larvae reared in the laboratory. J. exp. mar. Biol. Ecol. 167: 149–161.
Zar, J. H., 1984. Biostatistical Analysis. Prentice-Hall, Englewood Cliffs, NJ: 718 pp.
Zhukova, N. V. & N. A. Aizdaicher, 1995. Fatty acid composition of 15 species of marine microalgae. Phytochemistry 39(2): 351–356.
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Navarro, N., Yúfera, M. & García-Gallego, M. Use of freeze-dried microalgae for rearing gilthead seabream, Sparus aurata L., larvae. II. Biochemical composition. Hydrobiologia 452, 69–77 (2001). https://doi.org/10.1023/A:1011971810072
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DOI: https://doi.org/10.1023/A:1011971810072