Abstract
Shark DNA extracted from products used in trade(e.g. soup and dried fins) was amplified usingPCR. A strategy is described that permits theidentification of amplified material to species(for mtDNA Cytb and NADH2 loci) and thespecies-specific amplification of basking sharkDNA from mixed templates (for the Cytb locus). These methods will be useful for forensicapplications to govern trade in these heavilyexploited species.
References
Heist EJ,Gold JR (1999) Genetic identification of sharks in the US Atlantic large coastal shark fishery. Fish. Bull., 97, 53-61.
Heist EJ,Graves JE,Musick JA (1995) Population genetics of the sandbar shark (Carcharhinus plumbeus) in the Gulf of Mexico and mid-Atlantic Bight. Copea, 3, 555-562.
Heist EJ,Musick JA,Graves JE (1996) Mitochondrial DNA diversity and divergence among sharpnose sharks, Rhizoprionodon terraenovae, from the Gulf of Mexico and mid-Atlantic Bight. Fish. Bull., 94, 664-668.
Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nuceotide sequences. J. Mol. Evol., 16, 111-120.
Rose DA (1997) An Overview of World Trade in Sharks and Other Cartilaginous Fishes. Traffic Press, Cambridge.
Watts S (2000) Finned alive. BBC Wildlife, 18, 35-39.
Weaver LA,Lundstrom RC,Colbert AA (1999) Identification of shark species by polyacrylamide gel isoelectric focusing of sarcoplasmic proteins. J. AOAC Internat., 82, 1163-1170.
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Hoelzel, A. Shark fishing in fin soup. Conservation Genetics 2, 69–72 (2001). https://doi.org/10.1023/A:1011590517389
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DOI: https://doi.org/10.1023/A:1011590517389