Abstract
The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9 h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.
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Dolan A., Jamieson F.E., Cunninghain C., Barnett B.C., and McGeoch D.J., J Virol 72, 2010–2021, 1998.
Davis-Poynter N., Bell S., Minson T., and Browne H., J Virol 68, 7586–7590, 1994.
McGeoch D.J., Cunningham C., Mclntyre G., and Dolan A., J Gen Virol 72, 3057–3075, 1991.
Jacobson J.G., Martin S.L., and Coen D.M., Virology 63, 1839–1843, 1989.
Dezelee S., Bras F., Vende P., Simonet B., Nguyen X., Flamand A., and Masse M.J., Virus Res 42, 27–36, 1996.
Moore P.S., Gao S.-J., Dominguez G., Cesarman E., Lungu O., Knowles D.M., Garber R., Pellett P.E., McGeoch D.J., and Chang Y., J Virol 70, 549–558, 1996.
Kibler P.K., Duncan J., Keith B.D., Hupel T., and Smiley J.R., J Virol 65, 6749–6760, 1991.
Read G.S., Sharp J.A., and Summers W.C., Virology 138, 368–372, 1984.
Read G.S. and Summers W.C., Proc Natl Acad Sci USA 79, 5215–5219, 1982.
Cook W.J. and Coen D.M., Virology 218, 204–213, 1996.
Hann L.E., Cook W.J., Uprichard S.L., Knipe D.M., and Coen D.M., J Virol 72, 7709–7714, 1998.
Cook W.J., Wobbe K.K., Böni J., and Coen D.M., Virology 218, 193–203, 1996.
Jacobson J.G., Leib D.A., Goldstein D.J., Bogard C.L., Schaffer P.A., Weller S.K., and Coen D.M., Virology 173, 276–283, 1989.
Sanders P.G., Wilkie N.M., and Davison A.J., J Gen Virol 63, 277–295, 1982.
Rajcάni J. and Vojvodova A., Acta Virol 42, 103–118, 1998.
Ward P.L., Campadelli-Fiume G., Avitabile E., and Roizman B., J Virol 68, 7406–7417, 1994.
Tognon M., Guandalini R., Romanelli M.G., Manservigi R., and Trevisani B., Virus Res 18, 135–150, 1990.
Jacobson J.G., Chen S.-H., Cook W.J., Kramer M.F., and Coen D.M., Virology 242, 161–169, 1998.
Tsurumi T., Maeno K., and Nishiyama Y., J Biochem 99, 981–984, 1986.
Daikoku T., Shibata S., Goshima F., Oshima S., Tsurumi T., Yamada H., Yamashita Y., and Nishiyama Y., Virology 235, 82–93, 1997. Identification and Characterization of HSV-2 UL24 327
Shiba C., Daikoku T., Goshima F., Takakuwa H., Yamauchi Y., Koiwai O., and Nishiyama Y., J Gen Virol 81, 2397–2405, 2000.
Rexon F.J., Semin Virol 4, 135–144, 1993.
Steven A.C. and Spear P.G., in Burnet R., Chiu W. and Garcea R. (eds), Structural Biology of Viruses. Oxford University Press, New York, 1996.
Salmon B., Cunningham C., Davison A.J., Harris W.J., and Baines J.D., J Vriol 72, 3779–3788, 1998.
Ali M.A., Forghani B., and Cantin E.M.,Virology 216, 278–283, 1996.
Patel A.H. and MacLean J.B., Virology 206, 465–478, 1995.