Abstract
Glucose kinetics were investigated in subcutaneous tissue of rabbits, in which a percutaneous device was implanted. The device was used for collection of tissue fluid and as carrier of an amperometric glucose sensor. Changes in glycaemia were reflected in subcutaneous tissue fluid. However, a limited number of responses of the implanted sensors were observed. Histologic evaluation showed thin fibrous capsules surrounding the implants. Accumulations of inflammatory cells were observed inside the subcutaneous chamber. The experiments again showed that changes in blood glucose concentration can be measured in subcutaneous tissue fluid collected with a percutaneous device. Nevertheless, implanted glucose sensors could not reliably monitor these changes. Supported by our histological observations and sufficient in vitro performance, we suppose that the cellular reaction to the sensor plays an important role in this poor in vivo performance. In combination with adsorption of tissue fluid proteins, this results in a reversible deactivation of implanted sensors. The exact mechanisms involved in this process are currently unknown and need further investigation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
D. A. GOUGH and J. C. ARMOUR, Diabetes 44 (1995) 1005.
D. M. FRASER in ``Biosensors in the body'' (JohnWiley & Sons, Chichester, 1997) p. 1.
J. C. PICKUP and D. R. THEVENOT in ``Advances in biosensors'' (JAI Press, London, 1993) p. 201.
S. J. UPDIKE, M. SHULTS and B. EKMAN, Diabetes Care 5 (1982) 207.
U. FISCHER, Acta Anaesthesiol. Scand. 39 (1995) 21.
M. GERRITSEN, J. A. JANSEN, A. KROS, R. J. M. NOLTE and J. A. LUTTERMAN, J. Invest. Surg. 11 (1998) 163.
W. M. REICHERT and A. A. SHARKAWY in ``Handbook of biomaterials evaluation: scientific, technical, and clinical testing of implant materials'' edited by A. F. von Recum (Taylor & Francis, London, 1999) p. 439.
A. F. VON RECUM and J. B. PARK, CRC Crit. Rev. Bioeng. 5 (1981) 37.
J. A. JANSEN, Y. C. G. J. PAQUAY and J. P. C. M. VAN DER WAERDEN, J. Biomed. Mater. Res. 28 (1994) 1047.
Y. C. G. J. PAQUAY, J. E. DE RUIJTER, J. P. C. M. VAN DER WAERDEN and J. A. JANSEN, ibid. 28 (1994) 1321.
R. J. GEISE, J. M. ADAMS, N. J. BARONE and A. M. YACYNYCH, Biosensors & Bioelectronics 6 (1991) 151.
H. P. KIMMICH and F. KREUZER in ``Progress in respiration research, oxygen pressure recording in gases, fluids, and tissues'' (S. Karger, Basel, 1996) p. 101.
H. B. M. VAN DER LUBBE, C. P. A. T. KLEIN and K. DE GROOT, Stain Technology 63 (1988) 171.
C. P. A. T. KLEIN, Y. M. H. F. SAUREN, W. E. MODDERMAN and J. P. C. M. VAN DER WAERDEN, J. Applied Biomaterials 5 (1994) 369.
F. MOUSSY, D. J. HARRISON and R. V. RAJOTTE, Int. J. Artif. Organs 17 (1994) 88.
B. J. GILLIGAN, M. C. SHULTS, R. K. RHODES and S. J. UPDIKE, Diabetes Care 17 (1994) 882.
W. KERNER, M. KIWIT, B. LINKE, F. KECK, H. ZIER and E. F. PFEIFFER, Biosensors and Bioelectronics 8 (1993) 473.
D. MOATTI SIRAT, F. CAPRON, V. POITOUT, G. REACH, D. S. BINDRA, Y. ZHANG, G. S. WILSON and D. R. THEVENOT, Diabetologia 35 (1992) 224.
K. W. CHANG, S. AISENBERG, J. S. SOELDNER and J. M. HIEBERT, Trans. Am. Soc. Artif. Int. Organs 19 (1973) 352
S. ERTEFAI and D. A. GOUGH, J. Biomed. Eng. 11 (1989) 362.
S. J. UPDIKE, M. C. SHULTS, R. K. RHODES, B. J. GILLIGAN, J. O. LUEBOW and D. VON HEIMBURG, ASAIO J. 40 (1994) 157.
A. REMES and D. F. WILLIAMS, Biomaterials 13 (1992) 731.
B. RONNEBERGER, W. J. KAO, J. M. ANDERSON and T. KISSEL, J. Biomed. Mater. Res. 30 (1996) 31. 24. T. J. FAHEY, S. VAN DEVENTER, S. MORGELLO, B. SHERRY, W. G. JONES, G. T. SHIRES, K. J. TRACEY, J. P. MINEI and A. CERAMI, Cytokine 2 (1990) 92.
P. S. LEPPERT and J. A. FIX, Biomaterials 11 (1990) 46.
J. E. BERGSMA, F. R. ROZEMA, R. R. M. BOS, G. BOERING, W. C. DE BRUIJN and A. J. PENNINGS, J. Biomed. Mater. Res. 29 (1995) 173.
A. S. ERIKSSON and P. THOMSEN, ibid. 12 (1991) 827.
A. S. ERIKSSON, L. E. ERICSON and P. THOMSEN, J. Mater. Sci.: Mater. Med. 5 (1994) 269.
D. J. CLAREMONT, I. E. SAMBROOK, C. PENTON and J. C. PICKUP, Diabetologia 29 (1986) 817.
K. REBRIN, U. FISCHER, H. HAHN VON DORSCHE, T. VON WOETKE, P. ABEL and E. BRUNSTEIN, J. Biomed. Eng. 14 (1992) 33.
A. A. SHARKAWY, B. KLITZMAN, G. A. TRUSKEY and W. M. REICHERT, J. Biomed. Mater. Res. 37 (1997) 401.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gerritsen, M., Kros, A., Lutterman, J.A. et al. A percutaneous device as model to study the in vivo performance of implantable amperometric glucose sensors. Journal of Materials Science: Materials in Medicine 12, 129–134 (2001). https://doi.org/10.1023/A:1008965826397
Issue Date:
DOI: https://doi.org/10.1023/A:1008965826397