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Study of the 16S–23S ribosomal DNA internal spacer of Coxiella burnetii

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Abstract

The complete 16S–23S ribosomal DNA (rDNA) internal transcribed spacer (ITS) of 22 isolates of the obligate intracellular bacterium Coxiella burnetii, the agent of Q fever, were amplified by the polymerase chain reaction (PCR) and sequenced using an a utomated laser fluorescent DNA sequencer. The ITS measured 497 base pairs (bp) and encoded isoleucine-tRNA and alanine-tRNA. The comparison of the sequence alignments of the 22 C. burnetii strains revealed very high levels of sequence similitary (< 99%) although they had different geographic origins and phenotypic characteristics. Sequencing of the 16S–23S rDNA ITS of C. burnetii could be utilized for identification of the bacterium but is not applicable to studies of epidemiology, virulence and taxonomy.

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Authors and Affiliations

  1. Faculté de Médecine, Unité des Rickettsies, Marseille, France

    Andreas Stein & Didier Raoult

  2. Department of Bacteriology, National Institute of Hygiene, Warsaw, Poland

    Danuta Kruszewska

  3. Service d'Information Médicale, Hôpital Timone, Marseille, France

    Joanny Gouvernet

Authors
  1. Andreas Stein
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  2. Danuta Kruszewska
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  3. Joanny Gouvernet
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  4. Didier Raoult
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Stein, A., Kruszewska, D., Gouvernet, J. et al. Study of the 16S–23S ribosomal DNA internal spacer of Coxiella burnetii. Eur J Epidemiol 13, 471–475 (1997). https://doi.org/10.1023/A:1007389315808

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  • DOI: https://doi.org/10.1023/A:1007389315808

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