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Gene expression in soybean (Glycine max L.) roots exposed to low temperature: Isolation of cDNA clone

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Abstract

A low temperature (10 °C) stress, soybean(Glycine max L.) subtraction library, wasconstructed in a pBluescript vector usingPolymerase Chain Reaction (PCR) amplified cDNA forsubtractive hybridization. Southern blottinganalysis indicated that one clone cs18 fromthe subtraction library hybridized to the lowtemperature stress cDNA library. One hundredthousand plaques from the soybean low temperaturestress cDNA library were screened using the insertcs18. Four clones cs18-14,cs18-15, cs18-16 and cs18-17 wereconfirmed to contain inserts that could hybridize tothe cs18 probe. Northern blot analysisindicated that cs18 mRNA was highly expressedin roots but moderately so in leaves under lowtemperature. Sequence analysis of insert cs18revealed that it had a 76% homology with thesequences of the corresponding portion of glucosedehydrogenase from Thermoplasma acidophiliuand 62% homology with a genomic DNA ofArabidopsis thaliana.

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Authors and Affiliations

  1. Research Centre for Plant Growth and Development, School of Botany and Zoology, University of Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, Republic of South Africa

    Tao Liu & W.A. Cress

  2. Research Centre for Plant Growth and Development, School of Botany and Zoology, University of Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, Republic of South Africa

    J. van Staden

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  1. Tao Liu
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  2. J. van Staden
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  3. W.A. Cress
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Correspondence to J. van Staden.

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Liu, T., van Staden, J. & Cress, W. Gene expression in soybean (Glycine max L.) roots exposed to low temperature: Isolation of cDNA clone. Plant Growth Regulation 30, 247–251 (2000). https://doi.org/10.1023/A:1006361510242

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  • DOI: https://doi.org/10.1023/A:1006361510242

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