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Improved ethanol tolerance of Saccharomyces cerevisiae strains by increases in fatty acid unsaturation via metabolic engineering

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Abstract

To enhance the ethanol tolerance of Saccharomyces cerevisiae, the Arabidopsis thaliana FAD2 gene and/or the S. cerevisiae OLE1 gene were over-expressed in this yeast. The transformant over-expressing both these genes could not only synthesize dienoic fatty acids but also increased the unsaturated fatty acid content of membrane lipid and then showed the highest viability in the presence of 15% (v/v) ethanol.

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Authors and Affiliations

  1. Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, 226-8501, Japan

    Susumu Kajiwara

  2. Department of Chemical Engineering, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo, 152-8552, Japan

    Keiko Suga

  3. Central Laboratories for Key Technology, Kirin Brewery Co., Ltd, 1-13-5 Fukuura Kanazawa-ku, Yokohama, 236-0004, Japan

    Hidetaka Sone & Katsumi Nakamura

Authors
  1. Susumu Kajiwara
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  2. Keiko Suga
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  3. Hidetaka Sone
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  4. Katsumi Nakamura
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Kajiwara, S., Suga, K., Sone, H. et al. Improved ethanol tolerance of Saccharomyces cerevisiae strains by increases in fatty acid unsaturation via metabolic engineering. Biotechnology Letters 22, 1839–1843 (2000). https://doi.org/10.1023/A:1005632522620

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  • DOI: https://doi.org/10.1023/A:1005632522620

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