Abstract
Ants of the myrmicine tribe Attini live in symbiosis with a fungus that provides them with food. In return the ants maintain optimal growth conditions for the fungus, weed out competing microorganisms, and inhibit the growth conditions of these competitors by chemical means. We present a comprehensive analysis of metapleural gland secretions of Acromyrmex octospinosus, using a recently developed method for the analysis of polar compounds by gas chromatography and mass spectrometry. We show that the chemical identity and quantitative recovery of different compounds in the metapleural gland secretion depends upon the method used and the type of colony from which the samples are taken. In addition to the two compounds previously recorded in the metapleural gland secretion of Acromyrmex ants (indolacetic acid and myrmicacin), 20 new compounds were detected in the secretion of a random sample of workers from two laboratory colonies and two field colonies. These compounds span the whole range of carboxylic acids from acetic acid to the long-chain fatty acids but comprise also some alcohols, lactones, and keto acids. The possible function of this highly complex secretion mixture is discussed.
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REFERENCES
Attygalle, A. B., Siegel, B., Vostowsky, O., Bestmann, H. J., and Maschwitz, U. 1989. Chemical composition and function of metapleural gland secretion of the ant, Crematogaster deformis Smith (Hymenoptera: Myrmicinae). J. Chem. Ecol. 15:317–328.
Bass, M., and Cherrett, J. M. 1994. The role of leaf-cutting ant workers (Hymenoptera: Formicidae) in fungus garden maintenance. Ecol. Entomol. 19:215–220.
Beattie, A. J., Turnbull, C., Hough, T., Jobson, S., and Knox, R. B. 1985. The vulnerability of pollen and fungal spores to ant secretions: Evidence and some evolutionary implications. Am. J. Bot. 72(4):606–614.
Beattie, A. J., Turnbull, C., Hough, T., and Knox, R. B. 1986. Antibiotic production: A possible function for the metapleural glands of ants (Hymenoptera: Formicidae). Ann. Entomol. Soc. Am. 79:448–450.
Blum, M. S. 1981. Chemical Defenses of Arthropods. Academic Press, New York, 562 pp.
Bot, A. N. M., and Boomsma, J. J. 1996. Variable metapleural gland size-allometries in Acromyrmex leafcutter ants (Hymenoptera: Formicidae). J. Kans. Entomol. Soc. 69:375–383.
Boyd, N. D., and Martin, M. M. 1975. Faecal proteinases of the fungus-growing ant Atta texana: Properties, significance and possible origin. Insect Biochem. 5:619–635.
Brough, E. J. 1983. The antimicrobial activity of the mandibular gland secretion of a formicine ant, Calomyrmex sp. (Hymenoptera: Formicidae). J. Invert. Pathol. 42:306–311.
Brown, W. L. 1968. An hypothesis concerning the function of the metapleural glands in ants. Am. Nat. 102:188–191.
Cherrett, J. M., Powell, R. J., and Stradling, D. J. 1989. The mutualism between leaf-cutting ants and their fungus, pp. 93–120. Insect-Fungus Interactions. Royal Entomological Society of London, 14th Symposium, 1987, Academic Press, London.
Cowles, P. B. 1941. The germicidal action of the hydrogen ion and of the lower fatty acids. Yale J. Biol. Med. 13:571–578.
Craven, S. E., Dix, M. W., and Michaels, G. E. 1970. Attine fungus gardens contain yeasts. Science 169:184–186.
Currie, C. R., Scott, J. A., Summerbell, R. C., and Malloch, D. 1999a. Fungus-growing ants use antibiotic-producing bacteria to control garden parasites. Nature 398:701–704.
Currie, C. R., Mueller, U. G., and Malloch, D. 1999b. The agricultural pathology of ant fungus gardens. Proc. Natl. Acad. Sci. U.S.A. 96:7998–8002.
Dettner, K. 1984. Isopropylesters as wetting agents from the defensive secretion of the rove beetle Coprophilus striatulus F. (Coleoptera, Staphylinidae). Insect Biochem. 14:383–390.
Diehl-Fleig, and Valim-Labres, M. E. 1993. Fungi isolated from leaf-cutting ants Atta sexdens piriventris and Acromyrmex heyeri (Hymenoptera: Formicidae): Mucor spp. effects on Beauveria bassiana entomopathogen. Cienc. Cult. 45:142–144.
do Nascimento, R. R., Schoeters, E., Morgan, D. E., Billen, J., and Stradling, D. 1996. Chemistry of metapleural gland secretions of three attine ants, Atta sexdens rubropilosa, Atta cephalotes and Acromyrmex octospinosus (Hymenoptera: Formicidae). J. Chem. Ecol. 22:987–1000.
Dowd, P. F. 1992. Insect fungal symbionts: A promising source of detoxifying enzymes. J. Ind. Microbiol. 9:149–161.
Eisner, T., Meinwald, J., Monro, A., and Ghent, R. 1961. Defense mechanism of arthropods—I. The composition and function of the spray of the whipscorpion, Mastigoproctus giganteus (Lucas) (Arachnida, Pedipalpida). J. Insect Physiol. 6:272–298.
Fisher, P. J., Stradling, D. J., Sutton, B., and Petrini, C. L. E. 1996. Microfungi in the fungus gardens of the leaf-cutting ant Atta cephalotes: A preliminary study. Mycol. Res. 100:541–546.
Hervey, A., and Nair, M. S. R. 1979. Antibiotic metabolites of a fungus cultured by the gardening ants. Mycologia 71:1064–1066.
HÖlldobler, B., and Engel-Siegel, H. 1984. On the metapleural gland in ants. Psyche 91:201–224.
Iwanawi, Y. 1978. Myrmicacin, a new inhibitor for mitotic progression after metaphase. Protoplasma 95:267–271.
JaffÉ, K., and Puche, H. 1984. Colony-specific territorial marking with the metapleural gland secretion in the ant Solenopsis geminata (Fabr.). J. Insect Physiol. 30:265–270.
Knapp, J. J., Jackson, C. W., Howse, P. E., and Vilela, E. F. 1994. Mandibular gland secretions of leaf-cutting ants: Role in defence against alien fungi, p. 109, in A. Lenoir, G. Arnold, and M. Lepage (eds.). Les Insectes Sociaux, 12th Congress of the International Union for the Study of Social Insects, Paris, Université Paris.
Koidsumi, K. 1957. Antifungal action of cuticular lipids in insects. J. Insect Physiol. 1:40–51.
Kreisel, H. 1972. Pilze aus Pilzgärten von Atta insularis in Kuba. Z. Allg. Mikrobiol. 12:643–654.
Maile, R., Dani, F. R., Jones, G. R., Morgan, E. D., and Ortius, D. 1998. Sampling techniques for gas chromatographic-mass spectrometric analysis of long-chain free fatty acids from insect exocrine glands. J. Chromatogr. A 816:169–175.
Maschwitz, U. 1974. Vergleichende Untersuchungen zur Funktion der Ameisenmetathorakaldrüse. Oecologia 16:303–310.
Maschwitz, U., Koob, K., and Schildknecht, H. 1970. Ein Beitrag zur Funktion der Metathorakaldr üse der Ameisen. J. Insect. Physiol. 16:387–404.
Maurer, P., Debieu, D., Malosse, C., Leroux, P., and Riba, G. 1992. Sterols and symbiosis in the leaf-cutting ant Acromyrmex octospinosus (Reich) (Hymenoptera, Formicidae: Attini). Arch. Insect Biochem. Physiol. 20:13–21.
Morgan, E. D. 1990. Preparation of small-scale samples from insects for chromatography. Anal. Chim. Acta 236:227–235.
North, R. D., Jackson, C. W., and Howse, P. E. 1997. Evolutionary aspects of ant-fungus interactions in leaf-cutting ants. Tree 12:386–389.
Papa, J., and Papa, F. 1982. Inhibition des bacteries dans les nids d'Acromyrmex octospinosus Reich. Bull. Soc. Pathol. Exotique 75:415–425.
Pavan, M. 1958. Significato chimico e biologica di alcuni veleni di insetti. Inst. Anat. Comp. Univ. Pavia 1–75.
Powell, R. J., and Stradling, D. J. 1986. Factors influencing the growth of Attamyces bromatificus, a symbiont of attine ants. Trans. Br. Mycol. Soc. 87:205–213.
Quinlan, R. J., and Cherrett, J. M. 1977. The role of substrate preparation in the symbiosis between the leaf-cutting ant Acromyrmex octospinosus (Reich) and its food fungus. Ecol. Entomol. 2:161–170.
Rice, R. W. 1989. Analyzing tables of statistical tests. Evolution 43(1):223–225.
Schildknecht, H., and Koob, K. 1971. Myrmicacin, the first insect herbicide. Angew. Chem. 10:124–125.
Schoeters, E., and Billen, J. 1993. Anatomy and fine structure of the metapleural gland in Atta (Hymenoptera, Formicidae). Belg. J. Zool. 123:67–75.
Spoehr, H. A., Smith, J. H. C., Strain, H. H., Milner, H. W., and Hardin, G. J. 1949. Fatty Acid Antibacterials from Plants. Carnegie Institution of Washington Publications, Washington, D.C., pp. 36–68.
Veal, D. A., Trimble, J. E., and Beattie, A. J. 1992. Antimicrobial properties of secretions from the metapleural glands of Myrmecia gulosa (the Australian bull ant). J. Appl. Bacteriol. 72:188–194.
Weber, N. A. 1966. Fungus-growing ants. Science 153:587–604.
Wyss, O., Ludwig, B. J., and Joiner, R. R. 1945. The fungistatic and fungicidal action of fatty acids and related compounds. Arch. Biochem. 7:415–425.
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Ortius-Lechner, D., Maile, R., Morgan, E.D. et al. Metapleural Gland Secretion of the Leaf-cutter Ant Acromyrmex octospinosus: New Compounds and Their Functional Significance. J Chem Ecol 26, 1667–1683 (2000). https://doi.org/10.1023/A:1005543030518
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DOI: https://doi.org/10.1023/A:1005543030518