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Human Cholecystokinin-A Receptor Is Not an Oncofetal Protein

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Abstract

The CCK-A (cholecystokinin-A) receptor is selectively expressed by human pancreatic adenocarcinomas, suggesting a possible role in pancreatic tumorigenesis. In animals, pancreatic CCK receptor expression varies during ontogeny and neoplastic transformation. This study examined the temporal expression of CCK receptors in human fetal, postnatal, and adult pancreas to determine whether the appearance of CCK-A receptors in pancreatic adenocarcinomas reflected oncofetal antigen or pancreatic neoantigen expression. Messenger ribonucleic acid (mRNA) was isolated from six paraffin-embedded normal pancreatic autopsy specimens ranging in age from 17 weeks postfertilization through 26 days following full-term delivery, and samples of adult human tissues, including pancreas and pancreatic adenocarcinoma. Using reverse transcription-polymerase chain reactions, CCK-B receptor mRNA was expressed in all specimens of normal fetal and postnatal human pancreas, adult pancreas, and pancreatic adenocarcinomas. CCK-A receptor mRNA was selectively expressed only in pancreatic adenocarcinomas. These data suggest that selective CCK-A receptor expression in pancreatic adenocarcinomas reflects neoantigen expression in humans.

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Authors and Affiliations

  1. Divisions of Gastroenterology and Hepatology and of Clinical Pharmacology, Departments of Medicine and of Biochemistry and Molecular Pharmacology, Kimmel Cancer Center, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania

    David S. Weinberg, Chi So, Bruce Ruggeri, Sanjoy Biswas, Michael Barber & Scott Waldman

  2. Department of Pathology, Allegheny University of the Health Sciences, Philadelphia, Pennsylvania

    David S. Weinberg, Chi So, Bruce Ruggeri, Sanjoy Biswas, Michael Barber & Scott Waldman

Authors
  1. David S. Weinberg
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  2. Chi So
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  3. Bruce Ruggeri
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  4. Sanjoy Biswas
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  5. Michael Barber
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  6. Scott Waldman
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Weinberg, D.S., So, C., Ruggeri, B. et al. Human Cholecystokinin-A Receptor Is Not an Oncofetal Protein. Dig Dis Sci 45, 538–543 (2000). https://doi.org/10.1023/A:1005449308536

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