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Cloning and characterization of the durable tomato mosaic virus resistance gene Tm-22 from Lycopersicon esculentum

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Abstract

In tomato, infections by tomato mosaic virus are controlled by durable Tm-22 resistance. In order to gain insight into the processes underlying disease resistance and its durability, we cloned and analysed the Tm-22 resistance gene and the susceptible allele, tm-2. The Tm-22 gene was isolated by transposon tagging using a screen in which plants with a destroyed Tm-22 gene survive. The Tm-22 locus consists of a single gene that encodes an 861 amino acid polypeptide, which belongs to the CC-NBS-LRR class of resistance proteins. The putative tm-2 allele was cloned from susceptible tomato lines via PCR with primers based on the Tm-22 sequence. Interestingly, the tm-2 gene has an open reading frame that is comparable to the Tm-22 allele. Between the tm-2 and the Tm-22 polypeptide 38 amino acid differences are present of which 26 are located in the second half of the LRR-domain. Susceptible tomato plants, which were transformed with the Tm-22 gene, displayed resistance against ToMV infection. In addition, virus specificity, displayed by the Tm-22 resistance was conserved in these transgenic lines. To explain the durability of this resistance, it is proposed that the Tm-22-encoded resistance is aimed at the Achilles' heel of the virus.

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Authors and Affiliations

  1. Department Molecular Biology of Plants, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN, Haren, the Netherlands

    Frank C. Lanfermeijer, Jos Dijkhuis, Marcel J.G. Sturre & Jacques Hille

  2. Laboratory of Plant Physiology, Department of Plant Biology, University of Groningen, P.O. Box 14, 9750 AA, Haren, the Netherlands

    Frank C. Lanfermeijer

  3. Syngenta Seeds B.V., PO Box 2, Westeinde 62, 1600 AA, Enkhuizen, the Netherlands

    Peter de Haan

  4. Viruvation B.V., Wassenaarseweg 72, 2333 AL, Leiden, the Netherlands

    Peter de Haan

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  1. Frank C. Lanfermeijer
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  2. Jos Dijkhuis
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  5. Jacques Hille
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Correspondence to Frank C. Lanfermeijer.

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Lanfermeijer, F.C., Dijkhuis, J., Sturre, M.J. et al. Cloning and characterization of the durable tomato mosaic virus resistance gene Tm-22 from Lycopersicon esculentum . Plant Mol Biol 52, 1039–1051 (2003). https://doi.org/10.1023/A:1025434519282

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